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dc.contributor.authorLOBO, F. P.pt_BR
dc.contributor.authorYAMAGISHI, M. E. B.pt_BR
dc.contributor.authorCAETANO, A. R.pt_BR
dc.contributor.authorMCMANUS, C. M.pt_BR
dc.contributor.authorCARNEIRO, P. L.pt_BR
dc.contributor.authorFACO, O.pt_BR
dc.contributor.authorSOUZA, C. J. H.pt_BR
dc.contributor.authorPAIVA, S. R.pt_BR
dc.date.accessioned2014-01-02T11:11:11Zpt_BR
dc.date.available2014-01-02T11:11:11Zpt_BR
dc.date.created2014-01-02pt_BR
dc.date.issued2013pt_BR
dc.identifier.citationIn: INTERNATIONAL PLANT & ANIMAL GENOME, 21., 2013, San Diego. [Abstracts...]. [S.l.: s.n.], 2013.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/974765pt_BR
dc.descriptionThe origin and domestication footprints present in contemporary domestic sheep breeds are of fundamental importance to provide insights regarding the identification of major genetic pools and breeds of importance for conservation. To infer the origin of locally adapted Brazilian sheep, the complete mitochondrial sequence of six animals from three main breeds (Morada Nova, Santa Ines and Brazilian Creole) were assembled from data generated by the International Sheep Genome Consortium. Paired-end DNA sequences (100bp) were generated using a HiSeq2000 platform from shotgun libraries to a depth of genome coverage averaging 12X. mtDNA sequences were identified, aligned and assembled with the Burrows-Wheeler Alignment Tool, using NC_001941 as reference (coverage ranged from 400 to 800X per individual). An additional set of 16 complete mitochondrial genomes available at NCBI (HM236174 to HM236189) was used in the phylogenetic analysis. Consensus sequences were initially processed with the CSA software (Cyclic DNA Sequence Aligner) to rotate them to a common homologous region. Resulting sequences were aligned with prank, using default parameters for DNA sequences, trimmed and cleaned of gaps with Gblocks. Processed sequences were analyzed with Phylyp (1000 bootstraps) and Consence was used to generate a consensus tree from bootstrap results. Phy-fi online tool was used to draw the consensus tree. Obtained results confirm that all Brazilian sheep belong to the B Haplogroup (Europe). Further analyses are underway using mtDNA data generated by the Sheep Hapmap Consortium from a total of 80 animals representing 42 breeds from different regions of the world, to better evaluate the genetic relationship of Brazilian breeds with animals from Europe and Africa.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectGenômicapt_BR
dc.subjectSequência de DNApt_BR
dc.titleGenetic origin of Brazilian local adapted sheep (Ovis aries) breeds by complete mitochondrial genome data analysis.pt_BR
dc.typeResumo em anais e proceedingspt_BR
dc.date.updated2020-01-22T11:11:11Zpt_BR
dc.subject.thesagroOvelhapt_BR
dc.subject.nalthesaurusSheeppt_BR
dc.subject.nalthesaurusGenomicspt_BR
dc.subject.nalthesaurusMitochondrial DNApt_BR
dc.description.notesPôster P620.pt_BR
dc.format.extent2Não paginado.pt_BR
riaa.ainfo.id974765pt_BR
riaa.ainfo.lastupdate2020-01-22 -02:00:00pt_BR
dc.contributor.institutionFRANCISCO PEREIRA LOBO, CNPTIA; MICHEL EDUARDO BELEZA YAMAGISHI, CNPTIA; ALEXANDRE RODRIGUES CAETANO, CENARGEN; CONCEPTA M. MCMANUS, UFRGS; PAULO LUIS CARNEIRO, Universidade Estadual do Sudoeste da Bahia; OLIVARDO FACO, CNPC; CARLOS J. H. SOUZA, USDA ARS BARC; SAMUEL REZENDE PAIVA, Cenargen.pt_BR
Aparece en las colecciones:Resumo em anais de congresso (CNPTIA)

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