Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/979826
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorSILVA, M. C. M. dapt_BR
dc.contributor.authorSARTO, R. P. delpt_BR
dc.contributor.authorLUCENA, W. A.pt_BR
dc.contributor.authorRIGDEN, D. J.pt_BR
dc.contributor.authorTEIXEIRA, F. R.pt_BR
dc.contributor.authorBEZERRA, C. de A.pt_BR
dc.contributor.authorALBUQUERQUE, E. V. S.pt_BR
dc.contributor.authorGROSSI DE SA, M. F.pt_BR
dc.date.accessioned2017-03-22T23:48:19Z-
dc.date.available2017-03-22T23:48:19Z-
dc.date.created2014-02-13pt_BR
dc.date.issued2013pt_BR
dc.identifier.citationJournal of Biotechnology, v. 167, p. 377-385, 2013.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/979826pt_BR
dc.descriptionNumerous species of insect pests attack cotton plants, out of which the cotton boll weevil (Anthonomus grandis) is the main insect in Brazil and must be controlled to avert large economic losses. Like other insect pests, A. grandis secretes a high level of -amylases in the midgut lumen, which are required for digestion of carbohydrates. Thus, -amylase inhibitors (-AIs) represent a powerful tool to apply in the control of insect pests. Here, we applied DNA shuffling and phage display techniques and obtained a combinatorial library containing 108 ˛-AI variant forms. From this library, variants were selected exhibiting in vitro affinity for cotton boll weevil -amylases. Twenty-six variant sequences were cloned into plant expression vectors and expressed in Arabidopsis thaliana. Transformed plant extracts were assayed in vitro to select specific and potent -amylase inhibitors against boll weevil amylases. While the wild type inhibitors, used to create the shuffled library, did not inhibit the A. grandis -amylases, three -AI mutants, named -AIC3, -AIA11 and -AIG4 revealed high inhibitory activities against A. grandis -amylases in an in vitro assay. In summary, data reported here shown the potential biotechnology of new ˛-AI variant genes for cotton boll weevil control.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectAlfa-amylase inhibitorspt_BR
dc.subjectIn vitro molecular evolutionpt_BR
dc.titleEmploying in vitro directed molecular evolution for the selection of α-amylase variant inhibitors with activity toward cotton boll weevil enzyme.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-03-22T23:48:19Zpt_BR
dc.subject.thesagroAnthonomus Grandispt_BR
dc.subject.nalthesaurusDNA shufflingpt_BR
dc.subject.nalthesaurusinsect controlpt_BR
riaa.ainfo.id979826pt_BR
riaa.ainfo.lastupdate2017-03-22pt_BR
dc.contributor.institutionMARIA CRISTINA MATTAR DA SILVA, CENARGEN; RAFAEL PERSEGHINI DEL SARTO, UNB; WAGNER ALEXANDRE LUCENA, CNPA; DANIEL JOHN RIGDEN, UNIVERSITY OF LIVERPOOL, UK; FABÍOLA RODRIGUES TEIXEIRA, bolsista, CENARGEN; CAROLINE DE ANDRADE BEZERRA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ERIKA VALERIA SALIBA ALBUQUERQUE FR, CENARGEN; MARIA FATIMA GROSSI DE SA, CENARGEN.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CENARGEN)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
1s2.0S016816561300312Xmain.pdf1,68 MBAdobe PDFThumbnail
Visualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace