Título:	Identification of KCNJ11 as a functional candidate gene for bovine meat tenderness.
Autoria:	TIZIOTO, P. GASPARINI, G. SOUZA, M. M. MUDADU, M. de A. COUTINHO, L. L. MOURÃO, G. B. THOLON, P. MEIRELLES, S. L. C. TULLIO, R. R. ROSA, A. do N. ALENCAR, M. M. de MEDEIROS, S. R. SIQUEIRA, F. FEIJO, G. L. D. NASSU, R. T. REGITANO, L. C. de A.
Afiliação:	POLIANA TIZIOTO, Universidade Federal de São Carlos; GUSTAVO GASPARINI, POS-GRADUAÇÃO UFSCar/SÃO CARLOS, SP; MARCELA M. SOUZA, POS-GRADUAÇÃO UFSCar/SÃO CARLOS, SP; MAURICIO DE ALVARENGA MUDADU, CPPSE; LUIZ L. COUTINHO, PROF. ESALQ/PIRACICABA; GERSON B. MOURÃO, ESALQ/PIRACICABA; PATRICIA THOLON, CPPSE; SARAH L. C. MEIRELLES, PROF. UFLA/LAVRAS; RYMER RAMIZ TULLIO, CPPSE; ANTONIO DO NASCIMENTO ROSA, CNPGC; MAURICIO MELLO DE ALENCAR, CPPSE; SERGIO R. MEDEIROS, CAMPO GRANDE; FABIANE SIQUEIRA, CNPGC; GELSON LUIS DIAS FEIJO, CNPGC; RENATA TIEKO NASSU, CPPSE; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.
Ano de publicação:	2013
Referência:	Physiological Genomics, v. 45, n. 24, p. 1215-1221, 2013.
Conteúdo:	The potassium inwardly rectifying channel, subfamily J, member 11 (KCNJ11) gene was investigated as a candidate for meat tenderness based on the effects reported on muscle for KCNJ11 gene knockout in rat models and its position in a quantitative trait locus (QTL) for meat tenderness in the bovine genome. Sequence variations in the KCNJ11 gene were described by sequencing six amplified fragments, covering almost the entire gene. We identified single nucleotide polymorphisms (SNP) and validated them by different approaches, taking advantage of simultaneous projects that are being developed with the same Nelore population. By sequencing the KCNJ11 in Nelore steers representing extreme phenotypes for Warner-Bratzler shear force (WBSF), it was possible to identify 22 SNPs. We validated two of the identified markers by genotyping the whole population (n = 460). Analysis of association between genotypes and WBSF values revealed a significant additive effect of a SNP at different meat aging times (P ? 0.05). In addition, an association between the expression levels of KCNJ11 and WBSF was found, with lower expression levels of KCNJ11 associated with more tender meat (P ? 0.05). The results showed that the KCNJ11 gene is a candidate mapped to a QTL for meat tenderness previously identified on BTA15 and may be useful to identify animals with genetic potential to produce tender meat. The effect of KCNJ11 observed on muscle is potentially due to changes in activity of KATP channels, which in turn influence the flow of potassium in the intracellular space, allowing establishment of the membrane potential necessary for muscle contraction.
NAL Thesaurus:	beef meat
Palavras-chave:	Candidate gene Bovine Differential expression Molecular markers Warner Bratzler Shear force
Digital Object Identifier:	10.1152/physiolgenomics.00137.2012
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em anais de congresso (CPPSE)