Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/985516
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorMOURA, R. R.pt_BR
dc.contributor.authorSOUZA-FABJAN, J. M. G.pt_BR
dc.contributor.authorFONSECA, J. F. dapt_BR
dc.contributor.authorMELO, C. H. S.pt_BR
dc.contributor.authorSANCHEZ, D. J. D.pt_BR
dc.contributor.authorVIEIRA, M. P.pt_BR
dc.contributor.authorALMEIDA, T. M.pt_BR
dc.contributor.authorSEROVA, I. A.pt_BR
dc.contributor.authorSEROV, O. L.pt_BR
dc.contributor.authorPEREIRA, A. F.pt_BR
dc.contributor.authorTEIXEIRA, D. I. A.pt_BR
dc.contributor.authorMELO, L. M.pt_BR
dc.contributor.authorFREITAS, V. J. F.pt_BR
dc.date.accessioned2014-05-02T11:11:11Zpt_BR
dc.date.available2014-05-02T11:11:11Zpt_BR
dc.date.created2014-05-02pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationAnimal Reproduction, v. 11, n. 1, p. 37-43, Jan./Mar. 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/985516pt_BR
dc.descriptionAbstract: This study aimed to monitor estrous cycle parameters of a human granulocyte colony-stimulating factor (hG-CSF)-transgenic founder female goat and to perform superovulation and embryo recovery (surgical or transcervical method) for further transfer to recipients to quickly obtain offspring. Two experiments were performed using a transgenic (TF) and a non-transgenic (NTF) female. In experiment 1, three estrous cycles were monitored for the following parameters: estrus behavior, progesterone concentration and ovarian activity. In experiment 2, two superovulation/embryo recovery sessions were performed and the recovered embryos were transferred to previously prepared recipients. Data were compared by either t test or Fisher's exact test. The mean interval between natural estrus was 20.7 ± 0.6 and 19.7 ± 0.6 (P > 0.05) days for the TF and NTF, respectively. Progesterone concentrations and ovarian activity were normal and similar between goats. The ovulation rate was similar between TF and NTF (12.0 ± 1.4 vs. 18.0 ± 4.2 CL; P > 0.05). No significant differences in embryo recovery rate (P > 0.05) were observed between the surgical and transcervical methods for TF (69.2 vs. 72.7%) or NTF (100.0 vs. 86.7%). Sixteen embryos from the TF were transferred to recipients, and eight kids were born. Among these kids, the transgene was identified in three (two males and one female), resulting in a transgenesis rate of 37.5%. In summary, the TF is a true founder, since she proved fertility and capacity of transmitting the hG-CSF transgene to progeny, suggesting that the analyzed reproductive traits were not compromised by the presence of the transgene.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectTransgeniapt_BR
dc.subjectGoatpt_BR
dc.titleReproductive parameters and the use of MOET in transgenic founder goat carrying the human granulocyte colony-stimulating factor (hG-CSF) gene.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2014-05-02T11:11:11Zpt_BR
dc.subject.thesagroCaprinopt_BR
dc.subject.thesagroCabrapt_BR
dc.subject.thesagroTransferência de embriãopt_BR
dc.subject.thesagroHormônio animalpt_BR
dc.subject.thesagroProgesteronapt_BR
dc.subject.thesagroSuperovulaçãopt_BR
dc.subject.nalthesaurusEmbryo transferpt_BR
dc.subject.nalthesaurusProgesteronept_BR
dc.subject.nalthesaurusSuperovulationpt_BR
dc.subject.nalthesaurusTransgenesispt_BR
riaa.ainfo.id985516pt_BR
riaa.ainfo.lastupdate2014-05-02pt_BR
dc.contributor.institutionUniversidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; JEFERSON FERREIRA DA FONSECA, CNPC; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; Institute of Cytology and Genetics, Novosibirsk, Russia.; Institute of Cytology and Genetics, Novosibirsk, Russia.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPC)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
APIReproductiveparameters.pdf421,97 kBAdobe PDFThumbnail
Visualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace