Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/986801
Research center of Embrapa/Collection: Embrapa Uva e Vinho - Artigo em periódico indexado (ALICE)
Date Issued: 2014
Type of Material: Artigo em periódico indexado (ALICE)
Authors: NICKEL, O.
FAJARDO, T. V. M.
Additional Information: OSMAR NICKEL, CNPUV; THOR VINICIUS MARTINS FAJARDO, CNPUV.
Title: Detection of Viruses in Apples and Pears by Real Time RT-PCR Using 5'-Hydrolysis Probes
Publisher: Journal of Plant Pathology, v. 96, n. 1, p. 207-213, 2014.
Language: en
Keywords: RT-PCR
Description: Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV) and Apple mosaic virus (ApMV) are common in apples and pears and the main targets of virus elimination from propagation material. The objective of this work was to design primers and probes for a real time RT-PCR protocol for detection of the four above viruses. FAM/TAMRA-labeled probes and primers were designed by searching for highly conserved nucleotide regions in the coat protein gene of the four viruses. Infection levels in analyzed apple samples were 92.6, 96.4, 100 and 88% for ASGV, ASPV, ACLSV and ApMV, respectively. In pears, all pre-existing ASPV infections were detected. Viral infections were confirmed in a selection of commercial cultivars of apples and pear scions, and quince rootstocks, demonstrating the sensitivity and reliability of the designed primers and probes. Real time RT-PCR using 5'-labeled probes is suitable for checking sanitary quality as a routine test in certification programs.
Thesagro: Maça
Pera
Virus
DNA
NAL Thesaurus: Apple chlorotic leaf spot virus
Apple mosaic virus
Apple stem pitting virus
Apple stem grooving virus
Reverse transcriptase polymerase chain reaction
Fruit quality
Data Created: 2014-05-22
Appears in Collections:Artigo em periódico indexado (CNPUV)

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