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dc.contributor.authorBRANDÃO, L. N. S.pt_BR
dc.contributor.authorPITCHENIM, L. C.pt_BR
dc.contributor.authorMARUYAMA, F. H.pt_BR
dc.contributor.authorCHITARRA, C. S.pt_BR
dc.contributor.authorSILVA, G. F. R. dapt_BR
dc.contributor.authorKLEIN, C. S.pt_BR
dc.contributor.authorNAKAZATO, L.pt_BR
dc.contributor.authorDUTRA, V.pt_BR
dc.date.accessioned2014-09-09T11:11:11Zpt_BR
dc.date.available2014-09-09T11:11:11Zpt_BR
dc.date.created2014-09-09pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationPesquisa Veterinária Brasileira, Brasília, DF, v. 34, n. 7, p. 621-625, 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/994638pt_BR
dc.descriptionBased on diagnostic tests for the detection of nucleic acids without amplification through the use of gold nanoparticles (AuNPs) have been described for various diseases. This study aimed to develop a technique of unmodified AuNPs to detect Actinobacillus pleuropneumoniae (App). We used 70 lung samples from pigs, 17 with and 53 without characteristic lesions of pneumonia, to detect App. The primer used was based on ApxIV gene. The AuNPs test had a sensitivity of 93.8% and specificity of 84.6% when compared with PCR detection. The results showed good agreement between AuNPs and PCR testing, and the technique can be used as an alternative to conventional tests, since it is quick and easy, and does not require implementation infrastructure and skilled labor.pt_BR
dc.language.isoporpt_BR
dc.rightsopenAccesspt_BR
dc.subjectNanotecnologiapt_BR
dc.subjectAuNPpt_BR
dc.titlePadronização da técnica de nanopartícula de ouro não modificada (AuNPs) para detecção de Actinobacillus pleuropneumoniae em pulmões de suínos.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-05-20T11:11:11Zpt_BR
dc.subject.thesagroSanidade animalpt_BR
dc.subject.thesagroPneumoniapt_BR
dc.subject.thesagroSuínopt_BR
dc.subject.thesagroActinobacillus Pleuropneumoniaept_BR
dc.subject.nalthesaurusAnimal diseasespt_BR
dc.subject.nalthesaurusSwinept_BR
riaa.ainfo.id994638pt_BR
riaa.ainfo.lastupdate2016-05-20pt_BR
dc.contributor.institutionUFMT; LETÍCIA C. PITCHENIM, UFMT; FERNANDA H. MARUYAMA, UFMT; CRISTIANE S. CHITARRA, UFMT; GIVAGO F. R. da SILVA, UFMT; CATIA SILENE KLEIN, CNPSA; LUCIANO NAKAZATO, UFMT; VALÉRIA DUTRA, UFMT.pt_BR
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