Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/996859
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorSOUZA, A. L.pt_BR
dc.contributor.authorANGELO, P. C. da S.pt_BR
dc.contributor.authorNOGUEIRA, P. P. O.pt_BR
dc.contributor.authorGONÇALVES, J. F. C.pt_BR
dc.contributor.authorFRANCO, A. M.pt_BR
dc.contributor.authorASTOLFI-FILHO, S.pt_BR
dc.contributor.authorLÓPEZ-LOZANO, J. L.pt_BR
dc.contributor.authorANDRADE, E. V.pt_BR
dc.date.accessioned2014-10-09T08:18:15Z-
dc.date.available2014-10-09T08:18:15Z-
dc.date.created2014-10-08pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationGenetics and Molecular Research, v. 13, n. 3, p. 8014-8024, 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/996859pt_BR
dc.descriptionGuarana has great agricultural potential and is largely used therapeutically and in the production of non-alcoholic energy drinks. Genomic and proteomic studies are crucial to identify proteins that play central roles in the maintenance and viability of fruits, as well as to identify proteins related to the main metabolic pathways. However, the success of any protein analysis starts with the protein extract preparation, which needs to offer an extract that is free of contaminants. This study aimed to evaluate different extraction methods to obtain high-quantity and high-quality extracts that are compatible with analysis by 2-dimensional electrophoresis and tandem mass spectrometry protein identification. Three different methods were tested: trichloroacetic acid (TCA)/acetone, sodium dodecyl sulfate (SDS)/phenol, and polyvinylpolypyrrolidone (PVPP)/SDS/phenol.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectPlant proteomicspt_BR
dc.subjectTwo-dimensional electrophoresispt_BR
dc.titleMethod for obtaining high-resolution proteomic analysis from pericarps of guarana.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2014-10-09T08:18:15Zpt_BR
dc.subject.thesagroGuaranápt_BR
dc.subject.thesagroPaullinia Cupanapt_BR
dc.subject.nalthesauruspolyphenolspt_BR
riaa.ainfo.id996859pt_BR
riaa.ainfo.lastupdate2014-10-08pt_BR
dc.identifier.doihttp://dx.doi.org/10.4238/2014.September.29.14pt_BR
dc.contributor.institutionA. L. Souza, UFAM; PAULA CRISTINA DA SILVA ANGELO, CPAA; P. P. O. Nogueira, FIOCRUZ; J. F. C. Gonçalves, INPA; A. M. Franco, INPA; S. Astolfi-Filho, UFAM; J. L. López-Lozano, Fundação de Medicina Tropical do Amazonas; E. V. Andrade, UFAM.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CPAA)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
gmr4011.pdf894,44 kBAdobe PDFThumbnail
Visualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace