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dc.contributor.authorFAJARDO, T. V. M.pt_BR
dc.contributor.authorNICKEL, O.pt_BR
dc.date.accessioned2014-11-03T11:11:11Zpt_BR
dc.date.available2014-11-03T11:11:11Zpt_BR
dc.date.created2014-11-03pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationCiência Rural, Santa Maria, v. 44, n. 10, p. 1711-1714, out. 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/999019pt_BR
dc.descriptionThe viruses Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) are common in apples and pears and main targets of detection in propagation materials. This study aimed at demonstrating the usefulness of the hybridization method with a non-radioactive probe for simultaneous detection of these four viruses. The sensitivity of this method was suffi ciently high enabling the detection of ASGV, ACLSV, ASPV and ApMV in total RNA extracted from infected samples. The probe specifi city was confi rmed by reaction with homologous viral cDNA, individually cloned for each virus.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectASGVpt_BR
dc.subjectACLSVpt_BR
dc.subjectASPVpt_BR
dc.subjectApMVpt_BR
dc.subjectMacieirapt_BR
dc.subjectPolissondapt_BR
dc.titleSimultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2014-11-03T11:11:11Zpt_BR
dc.subject.thesagroMaçãpt_BR
dc.subject.thesagroDoença de plantapt_BR
dc.subject.thesagroHibridaçãopt_BR
dc.subject.nalthesaurusApple chlorotic leaf spot viruspt_BR
dc.subject.nalthesaurusApple mosaic viruspt_BR
dc.subject.nalthesaurusApple stem grooving viruspt_BR
dc.subject.nalthesaurusApple stem pitting viruspt_BR
riaa.ainfo.id999019pt_BR
riaa.ainfo.lastupdate2014-11-03pt_BR
dc.contributor.institutionTHOR VINICIUS MARTINS FAJARDO, CNPUV; OSMAR NICKEL, CNPUV.pt_BR
Aparece en las colecciones:Artigo em periódico indexado (CNPUV)

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