Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/999434
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorRODRIGUES, T. B.pt_BR
dc.contributor.authorKHAJURIA, C.pt_BR
dc.contributor.authorWANG, H.pt_BR
dc.contributor.authorMATZ, N.pt_BR
dc.contributor.authorCARDOSO, D. C.pt_BR
dc.contributor.authorVALICENTE, F. H.pt_BR
dc.contributor.authorZHOU, X.pt_BR
dc.contributor.authorSIEGFRIED, B.pt_BR
dc.date.accessioned2014-11-07T11:11:11Zpt_BR
dc.date.available2014-11-07T11:11:11Zpt_BR
dc.date.created2014-11-07pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationPlos One, San Francisco,v. 9, n. 10, p. 1-8, Oct. 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/999434pt_BR
dc.descriptionQuantitative Real-time PCR (qRT-PCR) is a powerful technique to investigate comparative gene expression. In general, normalization of results using a highly stable housekeeping gene (HKG) as an internal control is recommended and necessary. However, there are several reports suggesting that regulation of some HKGs is affected by different conditions. The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is a serious pest of corn in the United States and Europe. The expression profile of target genes related to insecticide exposure, resistance, and RNA interference has become an important experimental technique for study of western corn rootworms; however, lack of information on reliable HKGs under different conditions makes the interpretation of qRT-PCR results difficult. In this study, four distinct algorithms (Genorm, NormFinder, BestKeeper and delta-CT) and five candidate HKGs to genes of reference (b-actin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; b-tubulin; RPS9, ribosomal protein S9; EF1a, elongation factor-1a) were evaluated to determine the most reliable HKG under different experimental conditions including exposure to dsRNA and Bt toxins and among different tissues and developmental stages. Although all the HKGs tested exhibited relatively stable expression among the different treatments, some differences were noted. Among the five candidate reference genes evaluated, b-actin exhibited highly stable expression among different life stages. RPS9 exhibited the most similar pattern of expression among dsRNA treatments, and both experiments indicated that EF1a was the second most stable gene. EF1a was also the most stable for Bt exposure and among different tissues. These results will enable researchers to use more accurate and reliable normalization of qRT-PCR data in WCR experiments.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleValidation of reference housekeeping genes for gene expression studies in western corn rootworm (Diabrotica virgifera virgifera).pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-05-23T11:11:11Zpt_BR
dc.subject.thesagroGenept_BR
dc.subject.thesagroLagartapt_BR
dc.subject.thesagroGenéticapt_BR
dc.subject.thesagroPraga de plantapt_BR
dc.subject.nalthesaurusPlant pestspt_BR
riaa.ainfo.id999434pt_BR
riaa.ainfo.lastupdate2017-05-23pt_BR
dc.identifier.doi10.1371/journal.pone.0109825pt_BR
dc.contributor.institutionFERNANDO HERCOS VALICENTE, CNPMS.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPMS)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Validationreference.pdf360,72 kBAdobe PDFThumbnail
Visualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace