Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1008509
Research center of Embrapa/Collection: Embrapa Cerrados - Artigo em periódico indexado (ALICE)
Date Issued: 2014
Type of Material: Artigo em periódico indexado (ALICE)
Authors: SILVA, M. A. A.
SOUZA, M. M.
SILVA, G. S.
MELO, C. A. F.
CORRÊA, R. X.
ARAÚJO, I. S.
CONCEIÇÃO, L. D. H. C. S. da
Additional Information: LEO DUC HAA CARSON SCHWARTZHAUPT DA, CPAC.
Title: Analysis of transferability of microsatellite primers (SSR) in wild Passiflora species and intraspecific genetic diversity in Passiflora alata.
Publisher: Genetics and Molecular Research, v. 13, n. 3, p. 5908-5918, 2014.
Language: en
Keywords: Diversidade genética
Marcador microsatelite
Transferabilidade de primers
Description: ABSTRACT. The genus Passiflora L. is the most representative of Passifloraceae, with over 500 known species, among which 150-200 originated from Brazil. In addition to the great commercial importance of this genus for the fruit market, many of the species have exotic flowers with a huge diversity of colors and can thereby be exploited as ornamental plants. This study was aimed at investigating the transferability of microsatellite primers in wild Passiflora species (P. cacao, P. cincinnata, P. glandulosa, P. gibertii, and P. mucronata) and characterizing 29 P. alata accessions using microsatellite primers that were previously developed in a library enriched with microsatellites from P. edulis f. flavicarpa for P. alata. The interspecies cross-amplification rate varied, and P. cacao exhibited the highest rate of amplification, suggesting a greater degree of proximity to P. edulis. The study of intraspecific accessions in P. alata found genetic similarity, with values ranging from 0.47 to 1.00 and an average similarity of 0.74. Hence, this study revealed the intraspecific genetic variability of P. alata in the Universidade Estadual de Santa Cruz?s Active Germplasm Bank and will lead to the adoption of mating strategies between accessions; thus making their use more suitable for breeding purposes.
Thesagro: Marcador genético
Maracujá
Flor
Melhoramento genético vegetal
NAL Thesaurus: Passiflora alata
Genetic variation
Microsatellite repeats
DNA primers
Data Created: 2015-02-11
Appears in Collections:Artigo em periódico indexado (CPAC)

Files in This Item:
File Description SizeFormat 
AnalysisoftransferabilityLeo.pdf661,88 kBAdobe PDFThumbnail
View/Open

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace