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|Title:||Multi-Analytical approach reveals potential microbial indicators in soil for sugarcane model systems.|
|Authors:||NAVARRETE, A. A.|
DINIZ, T. R.
BRAGA, L. P. P.
SILVA, G. G. Z.
FRANCHINI, J. C.
EDWARDS, R. A.
TSAI, S. M.
|Affiliation:||ACACIO APARECIDO NAVARRETE, CENA; TATIANA ROSA DINIZ, CENA; LUCAS PALMA PEREZ BRAGA, CENA; GENIVALDO GUEIROS ZACARIAS SILVA, SAN DIEGO STATE UNIVERSITY; JULIO CEZAR FRANCHINI DOS SANTOS, CNPSO; RAFFAELA ROSSETTO, APTA; ROBERT ALAN EDWARDS, SAN DIEGO STATE UNIVERSITY - ARGONNE NATIONAL LABORATORY; SIU MUI TSAI, CENA.|
|Citation:||Plos One, [S. l.], Jun. 2015.|
|Description:||This study focused on the effects of organic and inorganic amendments and straw retention on the microbial biomass (MB) and taxonomic groups of bacteria in sugarcane-cultivated soils in a greenhouse mesocosm experiment monitored for gas emissions and chemical factors. The experiment consisted of combinations of synthetic nitrogen (N), vinasse (V; a liquid waste from ethanol production), and sugarcane-straw blankets. Increases in CO2-C and N2O-N emissions were identified shortly after the addition of both N and V to the soils, thus increasing MB nitrogen (MB-N) and decreasing MB carbon (MB-C) in the N+V- amended soils and altering soil chemical factors that were correlated with the MB. Across 57 soil metagenomic datasets, Actinobacteria (31.5%), Planctomycetes (12.3%), Deltaproteobacteria (12.3%), Alphaproteobacteria (12.0%) and Betaproteobacteria (11.1%) were the most dominant bacterial groups during the experiment. Differences in relative abun- dance of metagenomic sequences were mainly revealed for Acidobacteria, Actinobacteria, Gammaproteobacteria and Verrucomicrobia with regard to N+V fertilization and straw re- tention. Differential abundances in bacterial groups were confirmed using 16S rRNA gene- targeted phylum-specific primers for real-time PCR analysis in all soil samples, whose re- sults were in accordance with sequence data, except for Gammaproteobacteria. Actino- bacteria were more responsive to straw retention with Rubrobacterales, Bifidobacteriales and Actinomycetales related to the chemical factors of N+V-amended soils. Acidobacteria subgroup 7 and Opitutae, a verrucomicrobial class, were related to the chemical factors of soils without straw retention as a surface blanket. Taken together, the results showed that MB-C and MB-N responded to changes in soil chemical factors and CO2-C and N2O-N emissions, especially for N+V-amended soils. The results also indicated that several taxo- nomic groups of bacteria, such as Acidobacteria, Actinobacteria and Verrucomicrobia, and Competing Interests: The authors have declared that no competing interests exist. their subgroups acted as early-warning indicators of N+V amendments and straw retention in sugarcane-cultivated soils, which can alter the soil chemical factors.|
|Thesagro:||Cana de açúcar|
Microbiologia do solo
|Type of Material:||Separatas|
|Appears in Collections:||Artigo em periódico indexado (CNPSO)|
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