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|Research center of Embrapa/Collection:||Embrapa Clima Temperado - Artigo em periódico indexado (ALICE)|
|Type of Material:||Artigo em periódico indexado (ALICE)|
|Authors:||VARGAS, D. P.|
CORADIN, J. H.
FERREIRA, L. V.
DORNELES, A. O. S.
VALGAS, R. A.
PEREIRA, A. da S.
CASTRO, C. M.
DUTRA, L. F.
|Additional Information:||DAIANE PEIXOTO VARGAS|
LEONARDO FERREIRA DUTRA, CPACT.
JULIANA HEY CORADIN, CPACT
LETÍCIA VANNI FERREIRA
ATHOS ODIN SEVERO DORNELES, UFPEL
RICARDO ALEXANDRE VALGAS, CPACT
ARIONE DA SILVA PEREIRA, CPACT
CAROLINE MARQUES CASTRO, CPACT
|Title:||Osmoregulators on in vitro encapsulation of potato "BRS Clara" buds.|
|Publisher:||Plant Cell Culture and Micropropagation, v. 16, e155, 2020.|
|Description:||Synthetic or artificial seeds via encapsulated propagules could be used for in vitro regeneration and mass multiplication, for germplasm preservation and exchange of plant materials. An in vitro encapsulation of potato ?BRS Clara? buds efficient protocol was established. Lateral buds were immersed in a 4% sodium alginate matrix, dripped in CaCl2 for 20 minutes in different osmoregulatory agents: sucrose (8.72, 15.00, 21.28 and 30.00 g L-1) and mannitol (0.00, 11.63, 20.0, 28.37 and 40.0 g L-1). They were then held for 30 days at approximately 4°C and subsequently immersed in KNO3 for capsule decomplexing. Afterward, they were inoculated in MS medium with 3% sucrose and cultured in a growth room under irradiance of 43 μmol m-2 s-1, 16 hours-photoperiod and 25±2 °C temperature. Sucrose and mannitol at concentrations of 15 g L-1 and 20 g L-1, respectively, associated with the encapsulation matrix promoted 80% survival rate of ?BRS Clara? encapsulated buds.|
|Appears in Collections:||Artigo em periódico indexado (CPACT)|
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