Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1136424
Research center of Embrapa/Collection: Embrapa Mandioca e Fruticultura - Artigo em periódico indexado (ALICE)
Date Issued: 2021
Type of Material: Artigo em periódico indexado (ALICE)
Authors: GUERRA, P. A.
SOUZA, E. H.
MAX, D. A. S.
ROSS, M. L.
VILLALOBOS-OLIVERA, A.
LEDO, C. A. da S.
MARTINEZ-MONTERO, M. E.
SOUZA, F. V. D.
Additional Information: PATRÍCIA A. GUERRA; EVERTON H. SOUZA; DANIELA A.S. MAX; MÔNICA L. ROSS; ARIEL VILLALOBOS-OLIVERA; CARLOS ALBERTO DA SILVA LEDO, CNPMF; MARCOS E. MARTINEZ-MONTERO; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF.
Title: Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique.
Publisher: Anais da Academia Brasileira de Ciências, v.93, n.1, e20190555, [2021?]
Language: Ingles
Description: Cryopreservation of pineapple shoot tips has been established from various protocols, including droplet vitrification. Thus, this work aimed to evaluate the morphoanatomical conditions of the starting material over different times (30, 45 and 60 days) of culture before freezing and its correlation with the survival percentage of the cryopreserved shoot tips. Four accessions, Ananas comosus var. comosus (BGA-009); var. bracteatus (BGA-119); var. parguazensis (BGA-376), var. erectifolius (BGA-750) from the Pineapple Active Germplasm Bank (BGA Pineapple) and two hybrids from the Embrapa Genetic Breeding Program, FIB-ROX1 (var. bracteatus X var. erectifolius) and FIB-ROX2 (var. erectifolius X var. bracteatus), recently introduced in the field from in vitro storage, were used. Histological sections before freezing and the percentages of survival after freezing were obtained taking into account the different times of cultivation of the donor plants. The results showed a significative interaction between genotypes (accessions and hybrids) and the culture period. The accessions BGA-009 and BGA-119 showed the highest survival rates, with 95% and 90% respectively for the 30-day culture time. Different results were obtained for each genotype, showing the need for improvements in the standardization of starting material, which would allow better repeatability of the protocol.
Thesagro: Abacaxi
Data Created: 2021-11-22
ISSN: 1678-2690
Appears in Collections:Artigo em periódico indexado (CNPMF)

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