Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137644
Title: Genetic characterization of leaf and stripe rust resistance in the Brazilian wheat cultivar toropi.
Authors: ROSA, S. B.
ZANELLA, C. M.
HIEBERT, C. W.
BRULE-BABEL, A. L.
RANDHAWA, H. S.
SHORTER, S.
BOYD, L. A.
MCCALLUM, B. D.
BRAMMER, S. P.
Affiliation: SILVIA B. ROSA, CEROM, Centre de recherch ´ e sur les grains, 740 Chemin Trudeau, Saint-Mathieu-de-Beloeil, QC, J3G 0E2, Canada; CAMILA M. ZANELLA, 2 NIAB, Huntingdon Road, Cambridge, CB3 0LE, UK; COLIN W. HIEBERT, 3 Agriculture and Agri-Food Canada, Morden Research Centre, Morden, MB, R6M 1Y5, Canada; ANITA L. BRULE-BABEL, 4 University of Manitoba, 66 Dafoe Road, Winnipeg, MB, R3T 2N2, Canada; HARPINDER S. RANDHAWA, Agriculture and Agri-Food Canada, Lethbridge Research Centre, 5403-1 Avenue South, Lethbridge, AB, T1J 4P4, Canada; STEPHEN SHORTER, Plant and Food Research, Canterbury Agriculture & Science Centre, Gerald Street, Lincoln, New Zealand; LESLEY A. BOYD, 2 NIAB, Huntingdon Road, Cambridge, CB3 0LE, UK; BRENT D. MCCALLUM, Agriculture and Agri-Food Canada, Morden Research Centre, Morden, MB, R6M 1Y5, Canada; SANDRA PATUSSI BRAMMER, CNPT.
Date Issued: 2019
Citation: Phytopathology, v. 109, p. 1760-1768, 2019.
Description: Leaf and stripe rust are major threats to wheat production worldwide. The effective, multiple rust resistances present in the Brazilian cultivar Toropi makes it an excellent choice for a genetic study of rust resistance. Testing of DNA from different seed lots of Toropi with 2,194 polymorphic 90K iSelect single nucleotide polymorphism markers identified significant genetic divergence, with as much as 35% dissimilarity between seed lots. As a result, further work was conducted with a single plant line derived from Toropi variant Toropi-6.4. A double haploid population with 168 lines derived from the cross Toropi-6.4 × Thatcher was phenotyped over multiple years and locations in Canada, New Zealand, and Kenya, with a total of seven field trials undertaken for leaf rust and nine for stripe rust. Genotyping with the 90K iSelect array, simple sequence repeat and Kompetitive allelespecific polymerase chain reaction markers resulted in a genetic map of 3,043 cM, containing 1,208 nonredundant markers. Significant quantitative trait loci (QTL) derived from Toropi-6.4 were identified in multiple environments on chromosomes 1B (QLr.crc-1BL/QYr.crc-1BL), 3B (QLr.crc3BS), 4B (QYr.crc-4BL), 5A (QLr.crc-5AL and QYr.crc-5AL), and 5D (QLr.crc-5DS). The QTL QLr.crc-1BL/QYr.crc-1BL colocated with the multi-rust resistance locus Lr46/Yr29, while the QTL QLr.crc-5DS located to the Lr78 locus previously found in a wheat backcross population derived from Toropi. Comparisons of QTL combinations showed QLr.crc-1BL to contribute a significantly enhanced leaf rust resistance when combined with QLr.crc-5AL or QLr.crc-5DS, more so than when QLr.crc-5AL and QLr.crc5DS were combined. A strong dditive effect was also seen when the stripe rust resistance QTL QYr.crc-1BL and QYr.crc-5AL were combined. Keywords: adult plant resistance, durable resistance, leaf rust, stripe rust, QTL, wheat
NAL Thesaurus: Leaf rust
Stripe rust
Wheat
Keywords: Adult plant resistance
Durable resistance
QTL
DOI: https://doi.org/10.1094/PHYTO-05-19-0159-R
Type of Material: Artigo de periódico
Access: openAccess
Appears in Collections:Artigo em periódico indexado (CNPT)

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