Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1149287
Title: Transcriptome profiling of the resistance response of Musa acuminata subsp. burmannicoides, var. Calcutta 4 to Pseudocercospora musae.
Authors: PINHEIRO, T. D. M.
REGO, E. C. S.
ALVES, G. S. C.
FONSECA, F. C. de A.
COTTA, M. G.
ANTONINO, J. D.
GOMES, T. G.
AMORIM, E. P.
FERREIRA, C. F.
COSTA, M. M. do C.
GRYNBERG, P.
TOGAWA, R. C.
MILLER, R. N. G.
Affiliation: TATIANA DAVID MIRANDA PINHEIRO, Universidade de Brasília; ERICA CRISTINA SILVA REGO, Universidade de Brasília; GABRIEL SERGIO COSTA ALVES, Universidade de Brasília; FERNANDO CAMPOS DE ASSIS FONSECA, Instituto Federal de Goiás; MICHELLE GUITTON COTTA, Universidade de Brasília; JOSE DIJAIR ANTONINO, Universidade Federal Rural de Pernambuco; TAÍSA GODOY GOMES, Universidade de Brasília; EDSON PERITO AMORIM, CNPMF; CLAUDIA FORTES FERREIRA, CNPMF; MARCOS MOTA DO CARMO COSTA, Cenargen; PRISCILA GRYNBERG, Cenargen; ROBERTO COITI TOGAWA, Cenargen; ROBERT NEIL GERARD MILLER, Universidade de Brasília.
Date Issued: 2022
Citation: International Journal of Molecular Sciences, v. 23, 2022. 13589.
Description: Banana (Musa spp.), which is one of the world's most popular and most traded fruits, is highly susceptible to pests and diseases. Pseudocercospora musae, responsible for Sigatoka leaf spot disease, is a principal fungal pathogen of Musa spp., resulting in serious economic damage to cultivars in the Cavendish subgroup. The aim of this study was to characterize genetic components of the early immune response to P. musae in Musa acuminata subsp. burmannicoides, var. Calcutta 4, a resistant wild diploid. Leaf RNA samples were extracted from Calcutta 4 three days after inoculation with fungal conidiospores, with paired-end sequencing conducted in inoculated and non-inoculated controls using lllumina HiSeq 4000 technology. Following mapping to the reference M. acuminata ssp. malaccensis var. Pahang genome, differentially expressed genes (DEGs) were identified and expression representation analyzed on the basis of gene ontology enrichment, Kyoto Encyclopedia of Genes and Genomes orthology and MapMan pathway analysis. Sequence data mapped to 29,757 gene transcript models in the reference Musa genome. A total of 1073 DEGs were identified in pathogen-inoculated cDNA libraries, in comparison to non-inoculated controls, with 32% overexpressed. GO enrichment analysis revealed common assignment to terms that included chitin binding, chitinase activity, pattern binding, oxidoreductase activity and transcription factor (TF) activity. Allocation to KEGG pathways revealed DEGs associated with environmental information processing, signaling, biosynthesis of secondary metabolites, and metabolism of terpenoids and polyketides. With 144 up-regulated DEGs potentially involved in biotic stress response pathways, including genes involved in cell wall reinforcement, PTI responses, TF regulation, phytohormone signaling and secondary metabolism, data demonstrated diverse early-stage defense responses to P. musae. With increased understanding of the defense responses occurring during the incompatible interaction in resistant Calcutta 4, these data are appropriate for the development of effective disease management approaches based on genetic improvement through introgression of candidate genes in superior cultivars.
Thesagro: Musa Acuminata
NAL Thesaurus: Biotic stress
Disease resistance
Transcriptome
Keywords: Pseudocercospora musae
Sigatoka leaf spot
Language: Ingles
DOI: https://doi.org/10.3390/ijms232113589
Type of Material: Artigo de periódico
Access: openAccess
Appears in Collections:Artigo em periódico indexado (CNPMF)

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