Title:	Chromoplast associated proteins from color storage root diversity in cassava (Manihot esculenta Crantz).
Authors:	CARVALHO, L. J. C. B. AGOSTINI, M. A. V. BLOCH JUNIOR, C.
Affiliation:	LUIZ JOAQUIM CASTELO B CARVALHO, CENARGEN; MARCO ANTONIO VALLE AGOSTINI; CARLOS BLOCH JUNIOR, CENARGEN.
Date Issued:	2004
Citation:	In: IINTERNATIONAL SCIENTIFIC MEETING CASSAVA BIOTECHOLOGY NETWORK, 6., 2004, Cali. Celebrating cassava diversity: a contribution from EMBRAPA/LBB. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2004. 1CD-ROM. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 111). Organized by Luiz J. C. B. Carvalho.
Description:	Accumulation of b-carotene in underground storage organ might result from a variety of regulatory mechanism, including steps in the syntheses pathway and its end product sequestration in the chromoplast. Such mechanisms may be especially important in non-green tissues performing massive carotenoid biosynthesis, such as in some clones of cassava (Manihot esculenta Crantz) storage roots. Recently, in our laboratory, specific carotene form accumulation in the storage root of cassava has been observed to parallel the high protein content in the storage root due to the occurrence of a larger protein population difference in chromoplast-associated proteins when the color cassava was compared with white one. Surprisingly, the protein content correlated with high amount of a particular form of carotenoid presence. These observations are further reported in the present communication. Results so far indicated that total soluble protein content in the storage root of color cassava reach more than 40% the amount of protein commonly reported for white cassava. Two dimensional gel electrophoresis analyses indicated that the number of proteins of a chromoplast-enriched suspension is about three times in color cassava in comparison to the identical tissue fraction extract from the white cassava. Protein size exclusion chromatography of the chromoplast-enriched suspension displayed three groups of chromoplastassociated proteins coming in association with three distinct patterns of carotenoid accumulated in traditional clone of cassava. These complex sets of protein groups were further characterized and partially purified in analytic RP-HPLC to forward our understand of the mechanism of carotenoid sequestering protein and the high protein content in the storage root of traditional cassava from Amazon.
Keywords:	Manihot esculenta Crantz
Type of Material:	Artigo em anais e proceedings
Access:	openAccess
Appears in Collections:	Artigo em anais de congresso (CENARGEN)