Título:	Distribution and genetic diversity of tomato-infecting begomoviruses in Brazil.
Autoria:	RIBEIRO, S. G. AMBROZEVÍCIUS, L. P. ÁVILA, A. C. BEZERRA, I. C. CALEGARIO, R. F. FERNANDES, J. J. LIMA, M. F. MLLO, R. N. de ROCHA, H. ZERBINI, F. M.
Afiliação:	MIRTES FREITAS LIMA, CPATSA.
Ano de publicação:	2003
Referência:	Archives of Virology, New York, v. 148, p. 281-295, 2003.
Conteúdo:	Tomato-infecting begomoviruses have been reported throughout Brazil since the introduction of the B biotype of Bemisia tabaci. Here, we report a large scale survey on the distribution and genetic diversity of tomato-infecting begomoviruses. Tomato samples withtypical begomovirus symptoms were collected in seven different states, comprising the major tomato growing areas of the country. Viruses were detected by polymerase chain reaction (PCR) using universal primers for the genus Begomovirus. PCR-amplified fragments were cloned and sequenced. Based on sequence comparisons and phylogenetic analyses, at least seven previously undescribed species of begomoviruses were found. Four of the new viruses were found exclusively in the Southeastern states, two exclusively in the Northeastern states, and one was found in both regions. Sequence comparisons reveal strong evidence of recombination among the Brazilian begomoviruses.Together, the results indicate the existence of a high degree of pre-existing genetic diversity among tomato-infecting begomoviruses in Brazil and suggest that these viruses have emerged after being transferred from natural hosts to tomatoes, due to he introduction into Brazil of a novel polyfagous biotype of the whitefly vector.
Thesagro:	Tomate Doença Vírus
NAL Thesaurus:	Begomovirus
Palavras-chave:	Diversidade genética Geminivirus Tomato
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPATSA)