Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/489141
Research center of Embrapa/Collection: Embrapa Milho e Sorgo - Artigo em periódico indexado (ALICE)
Date Issued: 1998
Type of Material: Artigo em periódico indexado (ALICE)
Authors: PINTO, P. P.
RAPOSEIRAS, R.
MACEDO, A. M.
SELDIN, L.
PAVA, E.
SA, N. M. H.
Additional Information: EMBRAPA-CNPMS.
Title: Effects of high temperature on survival, symbiotic performance and genomic modifications of bean nodulating Rhizobium strains.
Publisher: Revista de Microbiologia, São Paulo, v. 29, n. 4, p. 295-300, 1998.
Language: en
Keywords: strains-
survival-
capacity-
cerrado-
cerrado-soils
characteristics-
DNA-
inoculation-
properties-
soil-
stress-
temperature-
treatment-
symbiosis-
genotypes-
Description: High temperatures can affect the survival, establishment and symbiotic properties of Rhizobium strains. Bean nodulating Rhizobium strains are considered particularly sensitive because on this strains genetic recombinations and/or deletions occur frequently, thus compromising the use of these bacteria as inoculants. Rhizobium tropici and R. leguminosarum bv. phaseoli strains isolated from cerrado soils were exposed to thermal stress and the strains' growth, survival and symbiotic relationships as well as alterations in their genotypic and phenotypic characteristics were analysed. After successive thermal shocks at 45 degrees C for four hours, survival capacity appeared to be strain-specific, independent of thermo-tolerance and was more apparent in R. tropici strains. Certain R. leguminosarum bv. phaseoli strains had significant alterations in plant dry weight and DNA patterns obtained by AP-PCR method. Rhizobium tropici strains (with the exception of FJ2.21) were more stable than R. leguminosarum bv. phaseoli strains because no significant phenotypic alterations were observed following thermal treatments and they maintained their original genotypic pattern after inoculation in plants..
Data Created: 2006-01-23
Appears in Collections:Artigo em periódico indexado (CNPMS)

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