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Title: Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants.
Authors: SOUZA, F. V. D.
CANTO, A. M. M. E.
SOUZA, A. da S.
COSTA, M. A. P. de C.
Affiliation: FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; Ana Maria Mascarenhas Eloy Canto, UFRB; ANTONIO DA SILVA SOUZA, CNPMF; Maria Angélica Pereira de Carvalho Costa, UFRB.
Date Issued: 2010
Citation: Revista Brasileira de Fruticultura, Jaboticabal, v. 32, n. 2, p. 612-617, jun. 2010.
Description: This work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC ± 1oC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation treatment, were cultivated in proliferation medium and the number of regenerated plants per treatment was evaluated at 60 days of cultivation. The treatment that promoted the best etiolation of plants also promoted the worst regeneration rates, demonstrating the residual effect of the auxin used in the previous step in the regeneration of plants of the pineapple hybrid evaluated.
Thesagro: Germoplasma
Melhoramento Genético Vegetal
NAL Thesaurus: Ananas comosus var. comosus
germplasm conservation
Keywords: In vitro
Plant regulators
Nodal segments
ISSN: 0100-2945
Notes: Disponível em:<>. Acesso em 28 out.2010.
Type of Material: Artigo de periódico
Access: openAccess
Appears in Collections:Artigo em periódico indexado (CNPMF)

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