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|Title:||ITS-rDNA phylogeny of Colletotrichum spp. causal agent of apple glomerella leaf spot.|
|Authors:||GIARETTA, D. R.|
COELHO, C. M. M.
GUIDOLIN, A. F.
DANTAS, A. C. de M.
GOMES, E. A.
|Affiliation:||Diorvania Ribeiro Giaretta, UDESC; Amauri Bogo, UDESC; Cleide Maria Medeiros Coelho, UDESC; Altamir Frederico Guidolin, UDESC; Adriana Cibele de Mesquita Dantas, UFSC; ELIANE APARECIDA GOMES, CNPMS.|
|Citation:||Ciência Rural, Santa Maria, v. 40, n. 4, p. 806-812, abr. 2010.|
|Description:||Several diseases have affected apple production, among them there is Glomerella leaf spot (GLS) caused by Colletotrichum spp. The first report of this disease in apple was in plants nearby citrus orchards in São Paulo State, Brazil. The origin of this disease is still not clear, and studies based on the molecular phylogeny could relate the organisms evolutionarily and characterize possible mechanisms of divergent evolution. The amplification of 5.8S-ITS (Internal Transcribed Spacer) of rDNA of 51 pathogenic Colletotrichum spp. isolates from apples, pineapple guava and citrus produced one fragment of approximately 600 bases pairs (bp) for all the isolates analyzed. The amplified fragments were cleaved with restriction enzymes, and fragments from 90 to 500bp were obtained. The sequencing of this region allowed the generation of a phylogenetic tree, regardless of their hosts, and 5 isolated groups were obtained. From the "in silico" comparison, it was possible to verify a variation from 93 to 100% of similarity between the sequences studied and the Genbank data base. The causal agent of GLS is nearly related (clustered) to isolates of pineapple guava and to the citrus isolates used as control.|
|Type of Material:||Artigo de periódico|
|Appears in Collections:||Artigo em periódico indexado (CNPMS)|