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Unidade da Embrapa/Coleção:: Embrapa Milho e Sorgo - Artigo em periódico indexado (ALICE)
Data do documento: 27-Out-2010
Tipo do Material: Artigo em periódico indexado (ALICE)
Autoria: GIARETTA, D. R.
DANTAS, A. C. de M.
Informaçães Adicionais: Diorvania Ribeiro Giaretta, UDESC; Amauri Bogo, UDESC; Cleide Maria Medeiros Coelho, UDESC; Altamir Frederico Guidolin, UDESC; Adriana Cibele de Mesquita Dantas, UFSC; ELIANE APARECIDA GOMES, CNPMS.
Título: ITS-rDNA phylogeny of Colletotrichum spp. causal agent of apple glomerella leaf spot.
Edição: 2010
Fonte/Imprenta: Ciência Rural, Santa Maria, v. 40, n. 4, p. 806-812, abr. 2010.
Idioma: en
Palavras-chave: Filogenia molecular
Goiabeira serrana
Árvore filogenética
Conteúdo: Several diseases have affected apple production, among them there is Glomerella leaf spot (GLS) caused by Colletotrichum spp. The first report of this disease in apple was in plants nearby citrus orchards in São Paulo State, Brazil. The origin of this disease is still not clear, and studies based on the molecular phylogeny could relate the organisms evolutionarily and characterize possible mechanisms of divergent evolution. The amplification of 5.8S-ITS (Internal Transcribed Spacer) of rDNA of 51 pathogenic Colletotrichum spp. isolates from apples, pineapple guava and citrus produced one fragment of approximately 600 bases pairs (bp) for all the isolates analyzed. The amplified fragments were cleaved with restriction enzymes, and fragments from 90 to 500bp were obtained. The sequencing of this region allowed the generation of a phylogenetic tree, regardless of their hosts, and 5 isolated groups were obtained. From the "in silico" comparison, it was possible to verify a variation from 93 to 100% of similarity between the sequences studied and the Genbank data base. The causal agent of GLS is nearly related (clustered) to isolates of pineapple guava and to the citrus isolates used as control.
Ano de Publicação: 2010
Aparece nas coleções:Artigo em periódico indexado (CNPMS)

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