Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/872580
Research center of Embrapa/Collection: Embrapa Recursos Genéticos e Biotecnologia - Artigo em periódico indexado (ALICE)
Date Issued: 2010
Type of Material: Artigo em periódico indexado (ALICE)
Authors: OLIVEIRA, E. J. de
AMORIM, V. B. O.
MATOS, E. L. S.
COSTA, J. T.
CASTELLEN, M. da S.
PADUA, J. G.
DANTAS, J. L. L.
Additional Information: EDER JORGE DE OLIVEIRA, CNPMF; Vanusia Batista Oliveira Amorim, Fapesb; Edneide Luciana Santiago Matos, UFRB; Juliana Leles Costa, UFRB; MILENE DA SILVA CASTELLEN, DPD; JULIANO GOMES PADUA, CENARGEN; JORGE LUIZ LOYOLA DANTAS, CNPMF.
Title: Polymorphism of microsatellite markers in papaya (Carica papaya L.).
Publisher: Plant Molecular Biology Reporter, New York, v. 28, n. 3, p. 519-530, 2010.
Language: en
Keywords: Microsatellites
Papaya
Marcadores molecular
Description: A set of 81 new microsatellite markers for Carica papaya L. previously identified by data mining using freely available sequence information from Genbank were tested for polymorphism using 30 germplasm accessions from the Papaya Germplasm Bank (PGM) at Embrapa Mandioca e Fruticultura Tropical (CNPMF) and 18 landraces. The data were used to estimate pairwise genetic distances between the genotypes. A neighbor-joining based dendrogram was used to define clusters and infer possible genetic structuring of the collection. Most microsatellites were polymorphic (73%), with an observed number of alleles per locus ranging from one to eleven. The levels of observed and expected heterozygosity for 51 polymorphic loci varied from 0.00 to 0.85 and from 0.08 to 0.82, averaging 0.19 and 0.59, respectively. Forty-four percent of microsatellites showed polymorphism information content (PIC) higher than 0.50. The compound microsatellites seem to be more informative than dinucleotide and trinucleotide repeats in average alleles per locus and PIC. Among dinucleotides, AG/TC or GA/CT repeat motifs exhibited more informativeness than TA/AT, GT/CA and TG/AC repeat motifs. The neighbor-joining analysis based on shared allele distance could differentiate all the papaya accessions and landraces as well as differences in their genetic structure. This set of markers will be useful for examining parentage, inbreeding and population structure in papaya.
Thesagro: Carica Papaya
Germoplasma
Mamão
NAL Thesaurus: breeding
germplasm
polymorphism
Data Created: 2011-01-10
Appears in Collections:Artigo em periódico indexado (CENARGEN)

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