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Title: Inheritance of coffee leaf rust resistance and identification of AFLP markers linked to the resistance gene.
Authors: BRITO, G. G. de
Affiliation: GIOVANI GREIGH DE BRITO, CNPA; EVELINE TEIXEIRA CAIXETA, SAPC; ANA PAULA GALLINA, Universidade Federal de Viçosa; EUNIZE MACIEL ZAMBOLIM, Universidade Federal de Viçosa; LAÉRCIO ZAMBOLIM, Universidade Federal de Viçosa; VALDIR DIOLA, Universidade Federal de Viçosa; MARCELO ELHERS LOUREIRO, Universidade Federal de Viçosa.
Date Issued: 2010
Citation: EUPHYTICA, v. 173, p. 255-264. 2010.
Pages: 255-264
Description: The most important disease of Coffea arabica is coffee leaf rust caused by the fungus Hemileia vastatrix. The purpose of this study was to characterize the inheritance of coffee resistance gene(s) to race II of this pathogen and to identify and map molecular markers linked to this trait. Different populations were used: F2 (160 plants), BCr (20), and BCs (135), derived from a cross between the resistant genotype Hı´brido de Timor UFV 427-15 and the susceptible cultivar Catuaı´ Amarelo UFV 2143-236 (IAC 30). The segregation analysis showed that the resistance of Hı´brido de Timor to race II of the H. vastatrix is conferred by a single dominant gene. The amplification of 176 AFLP (Amplified fragment length polymorphism) primer combinations using bulked segregant analysis (BSA) allowed the identification of three molecular markers linked to the resistance gene. Genetic mapping of these three markers in the F2 population indicated that they are distributed on both sides, flanking the resistance gene. The markers E.CTC/M.TTT405 and E.CGT/M.TGT 300 were found linked to the resistance gene at 8.69 cM (LOD 18.91) and 25.10 cM (LOD 5.37), respectively, while E.CCT/M.TTC230 was localized on the other side of the gene, at 20.50 cM (LOD 6.15). These markers are the first rust resistance markers identified in Hı´brido de Timor and can be useful for marker assisted selection in coffee breeding programs.
Keywords: Resistance gene
AFLP marker
Bulked segregant analysis
Coffee leaf rust
Type of Material: Artigo de periódico
Access: openAccess
Appears in Collections:Artigo em periódico indexado (SAPC)

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