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Research center of Embrapa/Collection: Embrapa Acre - Artigo em periódico indexado (ALICE)
Date Issued: 2011
Type of Material: Artigo em periódico indexado (ALICE)
Authors: SOUSA, A. C. B.
SOUZA, A. P. de
Additional Information: Adna Cristina Barbosa de Sousa, UNICAMP; RODOLFO GODOY, CPPSE; Danilo Augusto Sforça, UNICAMP; TATIANA DE CAMPOS, CPAF-AC; Maria Imaculada Zucchi, IAC; LIANA JANK, CNPGC; Anete Pereira de Souza, UNICAMP.
Title: Genetic diversity analysis among pigeonpea genotypes adapted to South American regions based on microsatellite markers.
Publisher: Scientia Agricola, Piracicaba, v. 68, n. 4, p. 431-439, July/Aug. 2011.
Language: en
Keywords: Inferência bayesiana
Bayesian inference
Sofware Structure
Análisis estadístico
Marcadores genéticos
Simulación por computadora
Variación genética.
Description: The pigeonpea [Cajanus cajan (L) Millspaugh] is one of the most important perennial legume crops utilized in the food, fodder, soil conservation, crop-livestock integrated systems, reclaiming of degraded pastures and symbiotic nitrogen fixation. Microsatellite markers were used to estimate the genetic diversity of 77 pigeonpea genotypes selected from the germplasm collections at Embrapa Cattle-Southeast and, to evaluate their transferability to Phaseolus vulgaris and Vigna unguiculata species. The number of alleles per locus ranged from 2 to12, with an average of 5.1 alleles. The PIC values ranged from 0.11 to 0.80 (average 0.49) and the D values from 0.23 to 0.91 (average 0.58). The averages of observed and expected heterozygosity were 0.25 and 0.47, respectively, showing a deficit in heterozygosity. A model-based Bayesian approach implemented in the software STRUCTURE was used to assign genotypes into clusters. A dendrogram was constructed based on the modified Roger?s genetic distances using a neighbor-joining method (NJ). A total of four clusters were assembled by STRUCTURE and a strong tendency of correspondence between the Bayesian clusters in the NJ tree was observed. The genetic distance ranged from 0.09 to 0.62 (average 0.37), showing a low genetic diversity in the pigeonpea genotypes. Transferability of pigeonpea-specific microsatellites revealed a cross-amplification and the presence of polymorphic alleles in P. vulgaris and V. unguiculata.
Thesagro: Guandu
Cajanus cajan
Variação genética
Marcador genético
Polimorfismo genético
Phaseolus vulgaris
Feijão de corda
Vigna unguiculata
Método estatístico
Modelo de simulação
NAL Thesaurus: Pigeon peas
Genetic variation
Genetic markers
Statistical analysis
Genetic polymorphism
Computer simulation.
Data Created: 2011-10-05
Appears in Collections:Artigo em periódico indexado (CPAF-AC)

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