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|Research center of Embrapa/Collection:||Embrapa Soja - Resumo em anais de congresso (ALICE)|
|Type of Material:||Resumo em anais de congresso (ALICE)|
O'ROURKE, J. A.
ABDELNOOR, R. V.
PEDLEY, K. F.
WHITHAM, S. A.
GRAHAM, M. A.
|Additional Information:||AGUIDA MORALES; JAMIE A.O'ROURKE, USDA-ARS; ALUÍZIO BORÉM, UFV; RICARDO VILELA ABDELNOOR, CNPSO; KERRY F. PEDLEY, USDA-ARS; STEVEN A. WHITHAM, IOWA STATE UNIVERSITY; MICHELLE A. GRAHAM, USDA-ARS.|
|Title:||Combining transcriptome analyses and virus induced gene silencing to identify genes in the Rpp4-mediated asian soybean rust resistance Pathway.|
|Publisher:||In: MOLECULAR & CELLULAR BIOLOGY OF TE SOYBEAN, 14., Des Moines, 2012. [Proceedings...]. Des Moines: Iowa State University, 2012.|
|Description:||Six Asian Soybean Rust (ASR) resistance loci have been identified and mapped in soybean genome: Rpp1 (Resistance to Phakopsora pachyrhizi 1), Rpp2, Rpp3, Rpp4, Rpp5 and Rpp6. Of particular interest is Rpp4, which has remained stable and confers resistance against Phakopsora pachyrhizi isolates from around the world. Sequencing of the region harboring Rpp4 in the susceptible cultivar Williams 82 (Wm82) and the resistant cultivar (PI459025B) genotype identified a cluster of CC-NBS-LRR resistance genes. Meyers et al. (2009) developed Virus Induced Gene Silencing constructs from the LRR regions of the Wm82 Rpp4 candidate genes to confirm that orthologous genes were responsible for resistance in the resistant parent (PI459025B). In this study, RNA samples extracted from the same Rpp4 LRR silenced and empty vector treated plants, also infected with P. pachyrhizi (described by Meyer et al., 2009) were compared using the GeneChip® Soybean Genome Array (Affymetrix®). Since the plant samples differed only in the expression of Rpp4, comparisons of these samples would identify genes downstream of Rpp4 in the signaling pathway. In total, 383 differentially expressed probes were identified, many with functions related to defense. While the time point analyzed was late in defense signaling, bioinformatic approaches were useful in characterizing the defense response and identify transcription factors regulating the response.|
Doença de planta
Plant diseases and disorders.
|Appears in Collections:||Resumo em anais de congresso (CNPSO)|