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Research center of Embrapa/Collection: Embrapa Semiárido - Artigo em periódico indexado (ALICE)
Date Issued: 2013
Type of Material: Artigo em periódico indexado (ALICE)
Authors: COSTA, S. R. da
Additional Information: S. R. da COSTA, Universidade Estadual de Feira de Santana, Feira de Santana, BA,; CARLOS ANTONIO FERNANDES SANTOS, CPATSA.
Title: Allelic database and divergence among Psidium accessions by using microsatellite markers.
Publisher: Genetics and Molecular Research, v. 12, n. 4, p. 6802-6812, 2013.
Language: en
Keywords: Variabilidade genética
Marcadores microssatélites
Banco ativo de germoplasma
Melhoramento genético
Description: This study aimed to investigate the genetic variability among guava accessions and wild Psidium species of the Embrapa Semiárido germplasm collection by using microsatellite loci to guide genetic resources and breeding programs, emphasizing crosses between guava and other Psidium species. DNA was extracted using the 2X CTAB method, and polymerase chain reaction products were analyzed on 6% denatured polyacrylamide gels stained with silver nitrate. The unweighted pair-group method using arithmetic average dendrogram generated from the distance matrix of the Jaccard coefficient for 183 alleles of 13 microsatellite loci was used for visualization of genetic similarity. The number of base pairs was estimated using inverse mobility method based on the regression of known-size products. Analysis of molecular variance was performed using total decomposition between and within guava accessions. The accessions showed similarity from 0.75 to 1.00, with the dendrogram presenting cophenetic value of 0.85. Five groups were observed: the first included guava accessions; the second, P. guineense accessions; the third, one accession of P. friedrichsthalianum; and the last 2 groups, P. cattleianum. The genetic similarity among P. guineense and some guava accessions were above 80%, suggesting greater possibility to obtain interspecies hybrids between these 2 species. The genetic variability between the accessions was considered to be high (?ST = 0.238), indicating that guava genetic variability is not uniformly distributed among the 9 Brazilian states from where the accession were obtained. Obtaining a greater number of accessions by Brazilian states is recommended in order to have greater diversity among the species.
Thesagro: Goiaba.
NAL Thesaurus: Breeding
Data Created: 2013-12-17
ISSN: 1676-5680
Appears in Collections:Artigo em periódico indexado (CPATSA)

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