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Research center of Embrapa/Collection: Embrapa Agrossilvipastoril - Artigo em periódico indexado (ALICE)
Date Issued: 2013
Type of Material: Artigo em periódico indexado (ALICE)
Authors: LANNA FILHO, R.
Additional Information: Roberto Lanna Filho, UFRGS; Ricardo Magela de Souza, UFLA; ANDERSON FERREIRA, CPAMT; Maria Carolina Quecine Verdi, USP-ESALQ; Eduardo Alves, UFLA; USP-ESALQ.
Title: Biocontrol activity of Bacillus against a GFP-marked Pseudomonas syringae pv. tomato on tomato phylloplane
Publisher: Australasian Plant Pathology, Holanda, v. 42, n. 6, p. 643-651, nov. 2013
Pages: 643-651
Language: en
Keywords: Bacterial Speck
Endophytic Bactéria.
Description: We report the biocontrol activity of the endophytic bacteria Bacillus pumilus and Bacillus amyloliquefacies against the plant pathogenic bacteriumPseudomonas syringae pv.tomato strain NS4 transformed with theGFPexpressing gene. P. s . pv. tomato strain NS4 was obtained from the transformation of P. s .pv.tomato wild-type strain NW with the plasmid pNKGFP containing GFP-cassette for chromosomal integration. The GFP-marked strain was tested for hypersensitivity and pathogenicity, as well as population studies on the phylloplane, to determine its epidemiology and survival. In all of the bioassays strain NS4 presented similar characteristics to the wild-type, and was hence chosen as the model strain for these studies with antagonistic endophytic bacterial strains. In the biocontrol experiments, tomato plants were preinoculated with the endophytic bacteria 4 days prior to inoculation withP. s .pv.tomatostrains. On the tomato phylloplane the P. s .pv.tomato (strains NW and NS4) populations were drastically reduced, and tomato leaves showed reduced numbers of bacterial speck lesions, comparable to the standard chemical treatment copper oxychloride. Additionally, under epifluorescence microscopy, few GFP-tagged cells of strain NS4 were observed colonizing important niches on the tomato phylloplane. However, leaves untreated with the antagonists presented a large number of GFP-tagged cell aggregates. Our results demonstrated that endophytic bacteria can also act efficiently on the biocontrol of bacterial speck when applied as a foliar spray on the leaves. In addition, we highlighted the use of GFP-marked strain NS4 as a model system to study biocontrol agent and pathogen interactions, and growth and development of the pathogen on the tomato leaf surface.
NAL Thesaurus: Bacillus pumilus
Bacillus amyloliquefaciens
green fluorescent protein.
biological control
Data Created: 2014-02-03
ISSN: 0815-3191
Appears in Collections:Artigo em periódico indexado (CPAMT)

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