Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/980419
Research center of Embrapa/Collection: Embrapa Recursos Genéticos e Biotecnologia - Artigo em periódico indexado (ALICE)
Date Issued: 2013
Type of Material: Artigo em periódico indexado (ALICE)
Authors: DALCIN, L.
SILVA, R. C.
PAULINI, F.
SILVA, B. D. M.
NEVES, J. P.
LUCCI, C. M.
Additional Information: UnB; UnB; UnB; BIANCA DAMIANI MARQUES SILVA, CENARGEN; UnB; UnB.
Title: Cytoskeleton structure, pattern of mitochondrial activity and ultrastructure of frozen or vitrified sheep embryos.
Publisher: Cryobiology, v.67, n. 2, p. 137-145, 2013.
Language: en
Keywords: Congelamento lento
Vitrificação
Microscopia eletrônica de transmissão
Microscopia confocal
Organelas
Description: Even though sheep embryo cryopreservation is a commonly used procedure the survival and pregnancy outcomes can vary greatly. This study investigated whether cryopreservation was causing subtle changes in ultrastructure, mitochondrial activity or cytoskeletal integrity. Sheep embryos were either slow cooled in 1.5 M EG (n = 22), or vitrified in 20% EG + 20% DMSO with 0.5 M sucrose in Open Pulled Straws (OPS) (n = 24). One hour after warming the cryopreserved embryos differed from control embryos in that they had no mitochondrial activity combined with cytoskeletal disorganization and large vesicles. Vitrified embryos also showed many points of cytoskeleton disruption. Ultrastructural alterations resulting from actin filaments disorganization were observed in both cryopreserved groups. This includes areas presenting no cytoplasmic organelles, Golgi complex located far from the nucleus and a decrease of specialized intercellular junctions. Additionally, large vesicles were observed in vitrified morulae and early blastocysts. The alterations after cryopreservation were proportional to embryo quality as assessed using the stereomicroscope. Even in the absence of mitochondrial activity, grade I and II cryopreserved embryos contained mitochondria with normal ultrastructure. Embryos classified as grade I or II in the stereomicroscope revealed mild ultrastructural alterations, meaning that this tool is efficient to evaluate embryos after cryopreservation.
Data Created: 2014-02-18
Appears in Collections:Artigo em periódico indexado (CENARGEN)

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