Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1007801
Research center of Embrapa/Collection: Embrapa Informática Agropecuária - Artigo em periódico indexado (ALICE)
Date Issued: 2014
Type of Material: Artigo em periódico indexado (ALICE)
Authors: CARDOSO, D. C.
MARTINATI, J. C.
GIACHETTO, P. F.
VIDAL, R. O.
CARAZZOLLE, M. F.
PADILHA, L.
GUERREIRO-FILHO, O.
MALUF, M. P.
Additional Information: DANIELLE C. CARDOSO, IAC; JULIANA C MARTINATI, IAC; POLIANA FERNANDA GIACHETTO, CNPTIA; RAMON O. VIDAL; MARCELO F. CARAZZOLLE; LILIAN PADILHA, SAPC; OLIVEIRO GUERREIRO-FILHO, IAC; MIRIAN PEREZ MALUF, SAPC.
Title: Large-scale analysis of differential gene expression in coffee genotypes resistant and susceptible to leaf miner-toward the identification of candidate genes for marker assisted-selection.
Publisher: BMC Genomics, London, v. 15, p. 1-20, 2014.
Language: en
Keywords: Microarranjo
Plant defense
Description: A successful development of herbivorous insects into plant tissues depends on coordination of metabolic processes. Plants have evolved complex mechanisms to recognize such attacks, and to trigger a defense response. To understand the transcriptional basis of this response, we compare gene expression profiles of two coffee genotypes, susceptible and resistant to leaf miner (Leucoptera coffella). A total of 22000 EST sequences from the Coffee Genome Database were selected for a microarray analysis. Fluorescence probes were synthesized using mRNA from the infested and non-infested coffee plants. Array hybridization, scanning and data normalization were performed using Nimble Scan® e ArrayStar® platforms. Genes with foldchange values +/-2 were considered differentially expressed. A validation of 18 differentially expressed genes was performed in infected plants using qRT-PCR approach.
Thesagro: Café
Coffea Arábica.
NAL Thesaurus: Leafminers
Genes
Microarray technology.
Data Created: 2015-02-05
Appears in Collections:Artigo em periódico indexado (CNPTIA)

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