Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1013427
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dc.contributor.authorCAMARGO, L. S. de A.
dc.contributor.authorOTERO, R. J.
dc.contributor.authorGONÇALVES, F. S.
dc.contributor.authorMUNK, M. P.
dc.contributor.authorSOUZA, E. D.
dc.contributor.authorIGUMA, L. T.
dc.contributor.authorTOLEDO, J. R.
dc.contributor.authorPARRA PEREIRA, N. C.
dc.contributor.authorQUINTAO, C. C. R.
dc.contributor.authorVIANA, J. H. M.
dc.date.accessioned2025-03-13T20:27:52Z-
dc.date.available2025-03-13T20:27:52Z-
dc.date.created2015-04-13
dc.date.issued2014
dc.identifier.citationTransgenic Research, v. 23, p. 204-205, 2014.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1013427-
dc.descriptionThe present study evaluated embryo development and transgene expression after lentiviral microinjection into perivitelline space of early zygotes (6 h post in vitro fertilization) or matured oocytes. In a preliminary experiment we evaluated the effect of short incubation period and of sperm concentration on embryo development. In vitro matured oocytes (n = 591, five repetitions) were co-incubated with 2 or 4 million sperm/mL for 6 or 20 h and cleavage and blastocyst rate were analyzed by Chi square. Higher (P\0.05) cleavage rate was obtained with 4 million sperm for 20 h whereas the blastocyst rate at day seven post-insemination was similar (P\0.05) between 4 million sperm for 6 and 20 h.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectOocitos
dc.subjectZigotos
dc.subjectBlastocitos
dc.titleEffect of lentiviral vectors microinjection into the perivitelline space of bovine early zygotes on embryo development and transgene expression.
dc.typeResumo em anais e proceedings
dc.description.notesEdição dos abstracts do 9º University of California at Davis Transgenic Animal Research Conference, 2014, Tahoe City.
riaa.ainfo.id1013427
riaa.ainfo.lastupdate2025-03-13
dc.contributor.institutionLUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; R. J. OTERO; F. S. GONÇALVES; M. P. MUNK; E. D. SOUZA; LILIAN TAMY IGUMA, CNPGL; J. R. TOLEDO; N. C. PARRA PEREIRA; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, CNPGL.
Appears in Collections:Resumo em anais de congresso (CNPGL)

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