Title:	Effect of lentiviral vectors microinjection into the perivitelline space of bovine early zygotes on embryo development and transgene expression.
Authors:	CAMARGO, L. S. de A. OTERO, R. J. GONÇALVES, F. S. MUNK, M. P. SOUZA, E. D. IGUMA, L. T. TOLEDO, J. R. PARRA PEREIRA, N. C. QUINTAO, C. C. R. VIANA, J. H. M.
Affiliation:	LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; R. J. OTERO; F. S. GONÇALVES; M. P. MUNK; E. D. SOUZA; LILIAN TAMY IGUMA, CNPGL; J. R. TOLEDO; N. C. PARRA PEREIRA; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, CNPGL.
Date Issued:	2014
Citation:	Transgenic Research, v. 23, p. 204-205, 2014.
Description:	The present study evaluated embryo development and transgene expression after lentiviral microinjection into perivitelline space of early zygotes (6 h post in vitro fertilization) or matured oocytes. In a preliminary experiment we evaluated the effect of short incubation period and of sperm concentration on embryo development. In vitro matured oocytes (n = 591, five repetitions) were co-incubated with 2 or 4 million sperm/mL for 6 or 20 h and cleavage and blastocyst rate were analyzed by Chi square. Higher (P\0.05) cleavage rate was obtained with 4 million sperm for 20 h whereas the blastocyst rate at day seven post-insemination was similar (P\0.05) between 4 million sperm for 6 and 20 h.
Keywords:	Oocitos Zigotos Blastocitos
Notes:	Edição dos abstracts do 9º University of California at Davis Transgenic Animal Research Conference, 2014, Tahoe City.
Type of Material:	Resumo em anais e proceedings
Access:	openAccess
Appears in Collections:	Resumo em anais de congresso (CNPGL)