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dc.contributor.authorVIANA, J. F. C.
dc.contributor.authorCARRIJO, J.
dc.contributor.authorFREITAS, C. G.
dc.contributor.authorPAUL, A.
dc.contributor.authorALCARAZ, J.
dc.contributor.authorLACORTE, C. C.
dc.contributor.authorMIGLIOLO, L.
dc.contributor.authorANDRADE, C. A.
dc.contributor.authorFALCAO, R.
dc.contributor.authorSANTOS, N. C.
dc.contributor.authorGONÇALVES, S.
dc.contributor.authorOTERO-GONZÁLES, A. J.
dc.contributor.authorKHADEMHOSSEINI, A.
dc.contributor.authorDIAS, S. C.
dc.contributor.authorFRANCO, O. L.
dc.date.accessioned2018-07-19T01:02:56Z-
dc.date.available2018-07-19T01:02:56Z-
dc.date.created2015-10-16
dc.date.issued2015
dc.identifier.citationNanoscale, v. 7, p. 6238-6246, 2015.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1026638-
dc.descriptionCandida albicans is a common human-pathogenic fungal species with the ability to cause several diseases including surface infections. Despite the clear difficulties of Candida control, antimicrobial peptides (AMPs) have emerged as an alternative strategy for fungal control. In this report, different concentrations of antifungal Cm-p1 (Cencritchis muricatus peptide 1) were electrospun into nanofibers for drug delivery. The nanofibers were characterized by mass spectrometry confirming the presence of the peptide on the scaffold. Atomic force microscopy and scanning electronic microscopy were used to measure the diameters, showing that Cm-p1 affects fiber morphology as well as the diameter and scaffold thickness. The Cm-p1 release behavior from the nanofibers demonstrated peptide release from 30 min to three days, leading to effective yeast control in the first 24 hours. Moreover, the biocompatibility of the fibers were evaluated through a MTS assay as well as ROS production by using a HUVEC model, showing that the fibers do not affect cell viability and only nanofibers containing 10% Cm-p1?PVA improved ROS generation. In addition, the secretion of pro-inflammatory cytokines IL-6 and TNF-? by the HUVECs was also slightly modified by the 10% Cm-p1?PVA nanofibers. In conclusion, the electrospinning technique applied here allowed for the manufacture of biodegradable biomimetic nanofibrous extracellular membranes with the ability to control fungal infection.
dc.language.isoporpt_BR
dc.rightsopenAccesspt_BR
dc.subjectFungos
dc.subjectControle de fungos
dc.subjectNanofibras
dc.titleAntifungal nanofibers made by controlled release of sea animal derived peptide.
dc.typeArtigo de periódico
dc.date.updated2018-07-19T01:02:56Zpt_BR
dc.subject.thesagroCândida Albicans
riaa.ainfo.id1026638
riaa.ainfo.lastupdate2018-07-18
dc.contributor.institutionJULIANE F. C. VIANA, UNB
dc.contributor.institutionJÉSSICA CARRIJO, UCBpor
dc.contributor.institutionCAMILA G. FREITAS, UCBpor
dc.contributor.institutionARGHYA PAUL, MASSACHUSETTS INSTITUTE OF TECHNOLOGYpor
dc.contributor.institutionJARIB ALCARAZ, UNIVERSIDADE FEDERAL DE PERNAMBUCOpor
dc.contributor.institutionCRISTIANO CASTRO LACORTE, CENARGENpor
dc.contributor.institutionLUDOVICO MIGLIOLO, UCBpor
dc.contributor.institutionCÉSAR A. ANDRADE, UNIVERSIDADE FEDERAL DE PERNAMBUCOpor
dc.contributor.institutionROSANA FALCAO, CENARGENpor
dc.contributor.institutionNUNO C. SANTOS, UNIVERSIDADE DE LISBOApor
dc.contributor.institutionSÓNIA GONÇALVES, UNIVERSIDADE DE LISBOApor
dc.contributor.institutionANSELMO J. OTERO-GONZÁLEZ, UNIVERSIDAD DE LA HABANApor
dc.contributor.institutionALI KHADEMHOSSEINI, MASSACHUSETTS INSTITUTE OF TECHNOLOGYpor
dc.contributor.institutionSIMONI C. DIAS, UCBpor
dc.contributor.institutionOCTÁVIO L. FRANCO, UNB.por
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