Title:	Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo.
Authors:	MONTE, A. P. O. do LOIOLA FILHO, J. B. SOUZA, T. T. dos S. MIRANDA, M. de S. MAGALHÃES, L. C. BARROS, C. H. S. C. SILVA, A. A. de A. SANTOS, A. O. GUIMARÃES, A. de S. L. COSTA, J. M. da S. SOUSA, P. H. F. de NOGUEIRA, D. M. CORDEIRO, M. F. LOPES JÚNIOR, E. S.
Affiliation:	ALANE PAINS OLIVEIRA DO MONTE, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO JOÃO BOSCO LOIOLA FILHO, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO THAIS THATIANE DOS SANTOS SOUZA, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO MAYARA DE SOUZA MIRANDA, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO LÍVIA CORREIA MAGALHÃES, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO CELSO HENRIQUE SOUZA COSTA BARROS, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO ANA ARLETE DE AMORIM SILVA, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO ADRIANO OLIVEIRA SANTOS, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO AIONNE DE SOUZA LEITE GUIMARÃES, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO JONATHAN MAIA DA SILVA COSTA, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO PEDRO HUMBERTO FÉLIX DE SOUSA, UNIVERSIDADE DO ESTADO DA BAHIA DANIEL MAIA NOGUEIRA, CPATSA MABEL FREITAS CORDEIRO, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO EDILSON SOARES LOPES JÚNIOR, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO.
Date Issued:	2015
Citation:	Semina: Ciências Agrárias, Londrina, v. 36, n. 6, p. 4257-4268, 2015.
Description:	This study aimed to evaluate the efficacy of adding sucrose in vitrification solution of ovine embryos produced in vivo. Forty Dorper ewes were selected and superovulated. Immediately prior to the embryo collection by laparotomy, a laparoscopy was erformed to verify the superovulatory response. The recovered flushing was followed by embryo evaluation and embryos were divided in two experimental groups where embryos from Control group were submitted to a traditional vitrification protocol and embryos from ucrose group to a modified vitrification protocol with sucrose. After warming, embryos were again divided regarding cryoprotectant removal (Indirect) or not (Direct). The embryo quality was classified as embryos of degrees I (excellent or good), II (regular), III (poor) and IV (dead or degenerate). It was also verified the homogeneity of mass, occurrence of embryonic mass retraction and rupture of pellucid zone. The results were expressed as percentages and were subjected to Chi-square test with P < 0.05. The embryos vitrified in the presence of sucrose had lower proportions of lower-quality embryos after warming (22.20 vs. 44.50%), higher percentages of homogeneous embryos after warming (63.89 vs. 38.89 %) while concerning other parameters there was no difference between these groups. It can be concluded that the addition of 0.4 M sucrose during vitrification improves the embryo quality.
Thesagro:	Produção animal Ovino Criopreservação Ovulação Embrião Animal Açúcar Ovelha
NAL Thesaurus:	Animal production Sucrose Cryopreservation Embryo (animal) Ewes Ovulation
Keywords:	Raça Dorper Múltipla ovulação
DOI:	10.5433/1679-0359.2015v36n6Supl2p4257
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPATSA)