Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1036144
Research center of Embrapa/Collection: Embrapa Semiárido - Artigo em periódico indexado (ALICE)
Date Issued: 2015
Type of Material: Artigo em periódico indexado (ALICE)
Authors: MONTE, A. P. O. do
LOIOLA FILHO, J. B.
SOUZA, T. T. dos S.
MIRANDA, M. de S.
MAGALHÃES, L. C.
BARROS, C. H. S. C.
SILVA, A. A. de A.
SANTOS, A. O.
GUIMARÃES, A. de S. L.
COSTA, J. M. da S.
SOUSA, P. H. F. de
NOGUEIRA, D. M.
CORDEIRO, M. F.
LOPES JÚNIOR, E. S.
Additional Information: ALANE PAINS OLIVEIRA DO MONTE, Mestranda da UNIVASF; JOÃO BOSCO LOIOLA FILHO, Mestrando da UNIVASF; THAIS THATIANE DOS SANTOS SOUZA, Mestranda da UNIVASF; MAYARA DE SOUZA MIRANDA, Mestranda da UNIVASF; LÍVIA CORREIA MAGALHÃES, Mestranda da UNIVASF; CELSO HENRIQUE SOUZA COSTA BARROS, Estudante da UNIVASF; ANA ARLETE DE AMORIM SILVA, Estudante da UNIVASF; ADRIANO OLIVEIRA SANTOS, Estudante da UNIVASF; AIONNE DE SOUZA LEITE GUIMARÃES, Estudante da UNIVASF; JONATHAN MAIA DA SILVA COSTA, Estudante da UNIVASF; PEDRO HUMBERTO FÉLIX DE SOUSA, Professor da Universidade do Estado da Bahia, UNEB, Juazeiro, BA; DANIEL MAIA NOGUEIRA, CPATSA; MABEL FREITAS CORDEIRO, Professora da UNIVASF; EDILSON SOARES LOPES JÚNIOR, Professor da UNIVASF.
Title: Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo.
Publisher: Semina: Ciências Agrárias, Londrina, v. 36, n. 6, p. 4257-4268, 2015.
Language: en
Notes: Suplemento 2.
Keywords: Raça Dorper.
Description: This study aimed to evaluate the efficacy of adding sucrose in vitrification solution of ovine embryos produced in vivo. Forty Dorper ewes were selected and superovulated. Immediately prior to the embryo collection by laparotomy, a laparoscopy was erformed to verify the superovulatory response. The recovered flushing was followed by embryo evaluation and embryos were divided in two experimental groups where embryos from Control group were submitted to a traditional vitrification protocol and embryos from ucrose group to a modified vitrification protocol with sucrose. After warming, embryos were again divided regarding cryoprotectant removal (Indirect) or not (Direct). The embryo quality was classified as embryos of degrees I (excellent or good), II (regular), III (poor) and IV (dead or degenerate). It was also verified the homogeneity of mass, occurrence of embryonic mass retraction and rupture of pellucid zone. The results were expressed as percentages and were subjected to Chi-square test with P < 0.05. The embryos vitrified in the presence of sucrose had lower proportions of lower-quality embryos after warming (22.20 vs. 44.50%), higher percentages of homogeneous embryos after warming (63.89 vs. 38.89 %) while concerning other parameters there was no difference between these groups. It can be concluded that the addition of 0.4 M sucrose during vitrification improves the embryo quality.
Thesagro: Produção animal
Ovino
Cana de Açúcar
Criopreservação
Ovulação.
NAL Thesaurus: Animal production
Sucrose
Data Created: 2016-02-04
Appears in Collections:Artigo em periódico indexado (CPATSA)

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