Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1045154
Research center of Embrapa/Collection: Embrapa Rondônia - Artigo em periódico indexado (ALICE)
Date Issued: 2016
Type of Material: Artigo em periódico indexado (ALICE)
Authors: SANTOS, M. R. A. dos
GUIMARÃES, M. C. M.
PAZ, E. S.
MAGALHÃES, G. M. O.
SOUZA, C. A.
SMOZINSKI, C. V.
NOGUEIRA, W. O.
Additional Information: MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-Rondonia; UNIR; UNIR; UNIR; Bolsista, Embrapa Rondônia; Bolsista, Embrapa Rondônia; UNIR.
Title: Induction and growth pattern of callus from Piper permucronatum leaves.
Publisher: Revista Brasileira de Plantas Medicinais, Botucatu, v. 18, n. 1, p.142-148, 2016.
Language: en
Keywords: Callogenesis
Reguladores de crescimento.
Description: Piper permucronatum is a perennial shrub, a medicinal plant native to the Amazon Rainforest. Traditionally, the tea of its leaves is used to combat menstrual and intestinal cramps, stomach pain, digestive problems, diarrhea, hemorrhage, and nausea. Its leaf?s essential oil is effective against Aedes aegypti larvae; its flavones and flavanones have a fungicidal effect against Clamidosporium cladosporioides and C. sphaerospermum; its hexanic extract is effective against Leishmania amazonensis. The objective of this study was to provide a protocol for callus induction from P. permucronatum leaves and an identification of the callus growth pattern, focusing on the deceleration phase, when the callus cells must be subcultured into liquid medium in order to produce a cell suspension cultures. Leaf explants were inoculated in a solid MS medium supplemented with factorial combinations of 2,4-D, BA, NAA and GA3. Callus formation was evaluated weekly until the 49th day. Subsequently, new explants were inoculated at the hormonal combination that resulted in the highest callus cell proliferation and, every seven days during a period of 70 days, samples were dried and weighed to determine the callus growth pattern. NAA and GA3 were not effective for callus induction. Combinations of 2,4-D and BA resulted in callus induction and proliferation. The highest percentage of callus induction was observed with the combination of 4.52 µM 2,4-D and 4.44 µM BA. The calluses thereby produced were friable and whitish. The callus growth pattern followed a sigmoid shape. The deceleration phase started on the 56th day of culture.
Thesagro: Calogênese
Piperaceae.
NAL Thesaurus: growth regulators.
Data Created: 2016-05-17
Appears in Collections:Artigo em periódico indexado (CPAF-RO)

Files in This Item:
File Description SizeFormat 
2016RBPMPiperpermucronatum.pdf270,15 kBAdobe PDFThumbnail
View/Open

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace