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dc.contributor.authorSILVA, C.pt_BR
dc.contributor.authorMARTINS, C. F.pt_BR
dc.contributor.authorCUMPA, H. C. B.pt_BR
dc.contributor.authorMENDONÇA, A.pt_BR
dc.contributor.authorFRANCO, M. M.pt_BR
dc.contributor.authorBAO, S.pt_BR
dc.date.accessioned2016-09-13T11:11:11Zpt_BR
dc.date.available2016-09-13T11:11:11Zpt_BR
dc.date.created2016-09-13pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationIn: INTERNATIONAL CONGRESS OF ANIMAL REPRODUCTION, 18., 2016, In Tours. Proceedings... In Tours, France: INCAR, 2016.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1052832pt_BR
dc.descriptionThe aim of this study was to assess the status of overall methylation of three cell types and their role in bovine cloning in the presence or absence of TSA. Amniotic fluid cells were obtained from pregnant bovine Gyr and cultured in Amniomax Complete II medium (Gibco). Biopsies of adipose tissue and ear skin were collected from the same calf after birth. The cells were isolated by explant and cultured in Dulbeccos Modified Eagle Medium (Gibco).eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectFluido amnióticopt_BR
dc.subjectTransferência nuclearpt_BR
dc.subjectTricostatina Apt_BR
dc.subjectNuclear transferpt_BR
dc.titleGlobal methylation of bovine cells derived from amniotic fluid, adipose tissue and skin fibroblastos and its use in nuclear transfer in the presence or absence of Trichostatin A.pt_BR
dc.typeResumo em anais e proceedingspt_BR
dc.date.updated2016-09-13T11:11:11Zpt_BR
dc.subject.thesagroMelhoramento genético animalpt_BR
dc.subject.thesagroBovinopt_BR
dc.subject.thesagroPelept_BR
dc.subject.thesagroMetilaçãoeng
dc.subject.nalthesaurusAmniotic fluideng
dc.subject.nalthesaurusMethylationeng
dc.subject.nalthesaurusCellseng
dc.subject.nalthesaurusAdipose tissueeng
dc.format.extent2p. 445.pt_BR
riaa.ainfo.id1052832pt_BR
riaa.ainfo.lastupdate2016-09-13pt_BR
dc.contributor.institutionCAROLINA SILVA, UNIVERSIDADE DE BRASÍLIApt_BR
dc.contributor.institutionCARLOS FREDERICO MARTINS, CPACeng
dc.contributor.institutionHEIDI CHRISTINA BESSLER CUMPA, CPACeng
dc.contributor.institutionANELISE MENDONÇA, CENARGENeng
dc.contributor.institutionMAURICIO MACHAIM FRANCO, CENARGENeng
dc.contributor.institutionSÔNIA BÁO, UNIVERSIDADE DE BRASÍLIA.eng
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