Title:	Global methylation of bovine cells derived from amniotic fluid, adipose tissue and skin fibroblastos and its use in nuclear transfer in the presence or absence of Trichostatin A.
Authors:	SILVA, C. MARTINS, C. F. CUMPA, H. C. B. MENDONÇA, A. FRANCO, M. M. BAO, S.
Affiliation:	CAROLINA SILVA, UNIVERSIDADE DE BRASÍLIA CARLOS FREDERICO MARTINS, CPAC HEIDI CHRISTINA BESSLER CUMPA, CPAC ANELISE MENDONÇA, CENARGEN MAURICIO MACHAIM FRANCO, CENARGEN SÔNIA BÁO, UNIVERSIDADE DE BRASÍLIA.
Date Issued:	2016
Citation:	In: INTERNATIONAL CONGRESS OF ANIMAL REPRODUCTION, 18., 2016, In Tours. Proceedings... In Tours, France: INCAR, 2016.
Pages:	p. 445.
Description:	The aim of this study was to assess the status of overall methylation of three cell types and their role in bovine cloning in the presence or absence of TSA. Amniotic fluid cells were obtained from pregnant bovine Gyr and cultured in Amniomax Complete II medium (Gibco). Biopsies of adipose tissue and ear skin were collected from the same calf after birth. The cells were isolated by explant and cultured in Dulbeccos Modified Eagle Medium (Gibco).
Thesagro:	Melhoramento genético animal Bovino Pele Metilação
NAL Thesaurus:	Amniotic fluid Methylation Cells Adipose tissue
Keywords:	Fluido amniótico Transferência nuclear Tricostatina A Nuclear transfer
Type of Material:	Resumo em anais e proceedings
Access:	openAccess
Appears in Collections:	Resumo em anais de congresso (CPAC)