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dc.contributor.authorSANCHES, C. C.pt_BR
dc.contributor.authorROSINHA, G. M. S.pt_BR
dc.contributor.authorGALVÃO, C. E.pt_BR
dc.contributor.authorFEIJO, G. L. D.pt_BR
dc.contributor.authorARAUJO, F. R.pt_BR
dc.contributor.authorSOARES, C. O.pt_BR
dc.date.accessioned2017-01-31T11:11:11Zpt_BR
dc.date.available2017-01-31T11:11:11Zpt_BR
dc.date.created2017-01-31pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationPesquisa Veterinária Brasileira, Brasília, DF, v. 36, n. 11, p. 1059-1066, nov. 2016.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1062269pt_BR
dc.descriptionOne of the alterations that occur in the PRNP gene in bovines is the insertion/deletion (indel) of base sequences in specific regions, such as indels of 12-base pairs (bp) in intron 1 and of 23- bp in the promoter region. The deletion allele of 23 bp is associated with susceptibility to bovine spongiform encephalopathy (BSE) as well as the presence of the deletion allele of 12 bp. In the present study, the variability of nucleotides in the promoter region and intron 1 of the PRNP gene was genotyped for the Angus, Canchim, Nellore and Simmental bovine breeds to identify the genotype profiles of resistance and/or susceptibility to BSE in each animal. Genomic DNA was extracted for amplification of the target regions of the PRNP gene using polymerase chain reaction (PCR) and specific primers. The PCR products were submitted to electrophoresis in agarose gel 3% and sequencing for genotyping. With the exception of the Angus breed, most breeds exhibited a higher frequency of deletion alleles for 12 bp and 23 bp in comparison to their respective insertion alleles for both regions. These results represent an important contribution to understanding the formation process of the Brazilian herd in relation to bovine PRNP gene polymorphisms.pt_BR
dc.languageenpt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectPRNPpt_BR
dc.subjectIndelpt_BR
dc.titlePolymorphisms in the prion protein gene of cattle breeds from Brazil.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-03-07T11:11:11Zpt_BR
dc.subject.thesagroDoença da vaca loucapt_BR
dc.subject.thesagroPolimorfismopt_BR
dc.subject.thesagroBovinopt_BR
dc.subject.nalthesaurusBrazilpt_BR
dc.subject.nalthesaurusBovine spongiform encephalopathypt_BR
dc.subject.nalthesaurusPrionspt_BR
dc.subject.nalthesaurusCattlept_BR
riaa.ainfo.id1062269pt_BR
riaa.ainfo.lastupdate2017-03-07pt_BR
dc.contributor.institutionCRISTIANE C. SANCHES, UFMS; GRACIA MARIA SOARES ROSINHA, CNPGC; CLEER E. GALVÃO, UFMS; GELSON LUIS DIAS FEIJO, CNPGC; FLABIO RIBEIRO ARAUJO, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC.pt_BR
Appears in Collections:Artigo em periódico indexado (CNPGC)

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