Title:	Polymorphisms in the prion protein gene of cattle breeds from Brazil.
Authors:	SANCHES, C. C. ROSINHA, G. M. S. GALVÃO, C. E. FEIJO, G. L. D. ARAUJO, F. R. SOARES, C. O.
Affiliation:	CRISTIANE C. SANCHES, UFMS; GRACIA MARIA SOARES ROSINHA, CNPGC; CLEER E. GALVÃO, UFMS; GELSON LUIS DIAS FEIJO, CNPGC; FLABIO RIBEIRO ARAUJO, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC.
Date Issued:	2016
Citation:	Pesquisa Veterinária Brasileira, Brasília, DF, v. 36, n. 11, p. 1059-1066, nov. 2016.
Description:	One of the alterations that occur in the PRNP gene in bovines is the insertion/deletion (indel) of base sequences in specific regions, such as indels of 12-base pairs (bp) in intron 1 and of 23- bp in the promoter region. The deletion allele of 23 bp is associated with susceptibility to bovine spongiform encephalopathy (BSE) as well as the presence of the deletion allele of 12 bp. In the present study, the variability of nucleotides in the promoter region and intron 1 of the PRNP gene was genotyped for the Angus, Canchim, Nellore and Simmental bovine breeds to identify the genotype profiles of resistance and/or susceptibility to BSE in each animal. Genomic DNA was extracted for amplification of the target regions of the PRNP gene using polymerase chain reaction (PCR) and specific primers. The PCR products were submitted to electrophoresis in agarose gel 3% and sequencing for genotyping. With the exception of the Angus breed, most breeds exhibited a higher frequency of deletion alleles for 12 bp and 23 bp in comparison to their respective insertion alleles for both regions. These results represent an important contribution to understanding the formation process of the Brazilian herd in relation to bovine PRNP gene polymorphisms.
Thesagro:	Doença da vaca louca Polimorfismo Bovino
NAL Thesaurus:	Brazil Bovine spongiform encephalopathy Prions Cattle
Keywords:	PRNP Indel
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPGC)