Title:	Agrobacterium-mediated genetic transformation of a tropical elite maize line.
Authors:	SOUZA, R. A. V. de ALVES, M. de C. CARNEIRO, N. P. BARROS, B. de A. BOREM, A. CARNEIRO, A. A.
Affiliation:	Rafaeli Aparecida Vieira de Souza, Universidade Federal de Viçosa; MEIRE DE CASSIA ALVES, CNPMS; NEWTON PORTILHO CARNEIRO, CNPMS; BEATRIZ DE ALMEIDA BARROS, CNPMS; Aluízio Borém, Universidade Federal de Viçosa; ANDREA ALMEIDA CARNEIRO, CNPMS.
Date Issued:	2017
Citation:	Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 17, p. 133-140, 2017.
Description:	ABSTRACT: The efficiency of maize transformation mediated by Agrobacterium tumefaciens is influenced by various factors. The aim of this study was to test the effect of different concentrations of N6 salts (50% ? half strength, and 100% - full strength) in the infection and cocultivation media on genetic transformation efficiency of the L3 tropical elite maize line. Immature embryos were transformed via A. tumefaciens harboring the binary vector pTF102 containing the genes uidA and BAR under control of the CaMV35S promoter. The efficiency of the transgenic events produced was 3% for full strength and 1.1% for half strength N6 salts. Although under a lower concentration of salts, GUS expression was stronger; under this concentration, regeneration was less efficient. Thus, the results showed that the presence of 100% N6 salts in the infection and cocultivation media favored genetic transformation of the L3 maize inbred line mediated by A. tumefaciens.
Thesagro:	Embriogênese Genética Bactéria
Keywords:	Concentração de sal Transformação genética
DOI:	10.1590/1984-70332017v17n2a20
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPMS)