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dc.contributor.authorTAMIETTI, M. S.
dc.contributor.authorMIDORIKAWA, G. E. O.
dc.contributor.authorMENDES, T. D.
dc.contributor.authorDAMASO, M. C. T.
dc.contributor.authorBOM, E. P. S.
dc.contributor.authorSILVA, A. S.
dc.contributor.authorGOTTSCHALK, L. M. F.
dc.contributor.authorNORONHA, E. F.
dc.contributor.authorFAVARO, L. C. de L.
dc.date.accessioned2017-12-08T23:19:28Z-
dc.date.available2017-12-08T23:19:28Z-
dc.date.created2017-10-09
dc.date.issued2017
dc.identifier.citationIn: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 21.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 12., 2017, Aracaju, SE. [Proceedings ...]. São Paulo: Associação Brasileira de Engenharia Química, 2017.
dc.identifier.issn2447-2816
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1076994-
dc.descriptionCarbon catabolite repression (CCR) is a mechanism by which microorganisms can utilize preferably highly energetic compounds over those of difficult degradation. For Trichoderma reesei, the protein that acts as repressor in the presence of glucose is CRE1. In this project, we aim to delete cre1 gene in Trichoderma harzianum CFAM-422 and obtain mutants with enhanced production of biomass degrading enzymes. Disruption of cre1 in T. harzianum CFAM-422 was performed by gene replacement of cre1 for hph (hygromycin B phosphotransferase) via homologous recombination. Hygromycin resistant mutants and parental strains enzyme production was evaluated in both inductive and repressive conditions in four different carbon sources. Enzymatic indexes (EI) were determined and compared. All genetically stable transformants showed increased enzymatic index under inductive conditions and modest inhibition under repressive conditions for most carbon sources, indicating that the deletion of cre1 in T. harzianum can be beneficial to cellulase and hemicellulase production with reduced product inhibition.
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectProcessos de hidrolise enzimática de biomassa
dc.subjectCarbon Catabolite Repression
dc.subjectCellulolytic and hemicellulolytic enzymes
dc.titleEnhancement of Trichoderma harzianum CFAM-422 for cellulase and hemicellulase production by deletion of the carbon catabolite repressor gene cre1.
dc.typeArtigo em anais e proceedings
dc.date.updated2017-12-08T23:19:28Zpt_BR
dc.subject.thesagroCelulasept_BR
dc.subject.thesagroTrichoderma Harzianumpt_BR
dc.description.notesSINAFERM; SHEB. 3 a 6 de setembro. Seção Trabalhos. Ref. 59019.
dc.format.extent2Não paginado.
riaa.ainfo.id1076994
riaa.ainfo.lastupdate2017-12-08
dc.contributor.institutionMARIANA S. TAMIETTI, UNB; GLÁUCIA E. O. MIDORIKAWA; THAIS DEMARCHI MENDES, CNPAE; MONICA CARAMEZ TRICHES DAMASO, CNPAE; ELBA P. S. BOM, UFRJ; AYLA S. SILVA, INT; LEDA MARIA FORTES GOTTSCHALK, CTAA; ELIANE F. NORONHA, UNB; LEIA CECILIA DE LIMA FAVARO, CNPAE.
Aparece en las colecciones:Artigo em periódico indexado (CTAA)

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