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dc.contributor.authorRIBEIRO, C. A. G.
dc.contributor.authorPINTO, M. de O.
dc.contributor.authorMACIEL, T. E. F.
dc.contributor.authorPASTINA, M. M.
dc.contributor.authorGOMES, E. G. de
dc.contributor.authorGUIMARAES, C. T.
dc.date.accessioned2017-11-10T23:21:31Z-
dc.date.available2017-11-10T23:21:31Z-
dc.date.created2017-11-10
dc.date.issued2017
dc.identifier.citationScientia Agricola, Piracicaba, v. 74, n. 2, p. 163-168, 2017.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1079407-
dc.descriptionThe development of efficient and low-cost genotyping methods is essential to precise genetic characterization of cultivars. Here, we present a system based on fluorescently labeled universal tail sequence primers (UTSP) to resolve microsatellite (SSR) markers as an alternative for molecular fingerprinting of maize. A set of 20 SSRs using the UTSP presented an average polymorphic information content of 0.84, which provided a probability of random identity ranging from 10−7 to 10−14, and a minimum exclusion power of 99.99998 % in a group of 48 tropical maize single-cross hybrids traded in Brazil. The genetic diversity analysis based on multidimensional scaling explained approximately 28 % of the total variance for the first two coordinates, tending to group the hybrids according to their respective origin. Additionally, this genotyping system presented a high distinctiveness capacity, which is widely recommended for genetic purity and fingerprinting analyses. Thus, this marker system has a strong potential as a tool for complementary analysis of distinguishability, uniformity and stability required for cultivar registration.
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectGenotipagem
dc.subjectCaracterização genética
dc.titleUniversal tail sequence-SSR applied to molecular characterization of tropical maize hybrids.
dc.typeArtigo de periódico
dc.date.updated2017-11-10T23:21:31Zpt_BR
dc.subject.thesagroZea mays
dc.subject.thesagroVariedade
riaa.ainfo.id1079407
riaa.ainfo.lastupdate2017-11-10
dc.identifier.doi10.1590/1678-992x-2016-0079
dc.contributor.institutionCarlos Alexandre Gomes Ribeiro, Universidade Federal de Viçosa; MARCOS DE OLIVEIRA PINTO, CNPMS; Talles Eduardo Ferreira Maciel, Universidade Federal de Viçosa; MARIA MARTA PASTINA, CNPMS; Everaldo Gonçalves de Barros, Universidade Católica de Brasília; CLAUDIA TEIXEIRA GUIMARAES, CNPMS.
Aparece nas coleções:Artigo em periódico indexado (CNPMS)

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