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dc.contributor.authorOLARTE, L. C.
dc.contributor.authorDUARTE, L. de F. C.
dc.contributor.authorLEGUIZAMON, G. O. de C.
dc.contributor.authorARAUJO, F. R.
dc.contributor.authorVERBISCK, N. V.
dc.date.accessioned2017-11-10T23:25:22Z-
dc.date.available2017-11-10T23:25:22Z-
dc.date.created2017-11-10
dc.date.issued2016
dc.identifier.citationIn: IBERO-AMERICAN, 1.; BrMASS CONFERENCE ON MASS SPECTROMETRY, 6., 2016, Rio de Janeiro. Resumos... Campinas: Sociedade Brasileira de Espectrometria de Massas, 2016.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1079459-
dc.descriptionBovine tuberculosis is a zoonotic disease caused by the Mycobacterium bovis bacillum, which accounts for significant economic losses in cattle production besides being a public health threat. Our purpose is to establish MALDI-TOF (Matrix Assisted Laser Desorption Ionization –Time-of-Flight) mass spectrometry method to identify and classify mycobacteria species, by means of building a specific biomarker library after solid media culture for these microrganims. We have identified 26 different protocols employing MALDI-TOF to identify and classify mycobacteria from solid media culture within 58 articles published since 2010. Four consensus protocols were designed and our results lead to the following cell treatment conditions before MALDI-TOF analysis: heat inactivation at 95ºC for 45 minutes, disruption with zirconia beads and MagNA lyser (Roche) and formic acid 70% and acetonitrile protein extraction.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectMALDI-TOF mass spectrometrypt_BR
dc.titleIdentification and classification of Mycobacterium by MALDI-TOF mass spectrometry.
dc.typeResumo em anais e proceedings
dc.date.updated2017-11-13T11:11:11Zpt_BR
dc.subject.thesagroMycobacterium Bovispt_BR
dc.subject.nalthesaurusMycobacterium
dc.subject.nalthesaurusBovine tuberculosis
dc.subject.nalthesaurusSpecies identification
dc.subject.nalthesaurusMycobacterium smegmatis
riaa.ainfo.id1079459
riaa.ainfo.lastupdate2017-11-13 -02:00:00
dc.contributor.institutionLARISSA COSTA OLARTE, UFMS - Federal University of Mato Grosso do Sul (Campo Grande - MS - Brasil); LARISSA DE FÁTIMA CARDOSO DUARTE, UFMS - Federal University of Mato Grosso do Sul (Campo Grande - MS - Brasil); GISELE OLIVAS DE CAMPOS LEGUIZAMON, CNPGC; FLABIO RIBEIRO ARAUJO, CNPGC; NEWTON VALERIO VERBISCK, CNPGC.
Aparece en las colecciones:Resumo em anais de congresso (CNPGC)

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