Title:	Identification and classification of Mycobacterium by MALDI-TOF mass spectrometry.
Authors:	OLARTE, L. C. DUARTE, L. de F. C. LEGUIZAMON, G. O. de C. ARAUJO, F. R. VERBISCK, N. V.
Affiliation:	LARISSA COSTA OLARTE, UFMS - Federal University of Mato Grosso do Sul (Campo Grande - MS - Brasil); LARISSA DE FÁTIMA CARDOSO DUARTE, UFMS - Federal University of Mato Grosso do Sul (Campo Grande - MS - Brasil); GISELE OLIVAS DE CAMPOS LEGUIZAMON, CNPGC; FLABIO RIBEIRO ARAUJO, CNPGC; NEWTON VALERIO VERBISCK, CNPGC.
Date Issued:	2016
Citation:	In: IBERO-AMERICAN, 1.; BrMASS CONFERENCE ON MASS SPECTROMETRY, 6., 2016, Rio de Janeiro. Resumos... Campinas: Sociedade Brasileira de Espectrometria de Massas, 2016.
Description:	Bovine tuberculosis is a zoonotic disease caused by the Mycobacterium bovis bacillum, which accounts for significant economic losses in cattle production besides being a public health threat. Our purpose is to establish MALDI-TOF (Matrix Assisted Laser Desorption Ionization –Time-of-Flight) mass spectrometry method to identify and classify mycobacteria species, by means of building a specific biomarker library after solid media culture for these microrganims. We have identified 26 different protocols employing MALDI-TOF to identify and classify mycobacteria from solid media culture within 58 articles published since 2010. Four consensus protocols were designed and our results lead to the following cell treatment conditions before MALDI-TOF analysis: heat inactivation at 95ºC for 45 minutes, disruption with zirconia beads and MagNA lyser (Roche) and formic acid 70% and acetonitrile protein extraction.
Thesagro:	Mycobacterium Bovis
NAL Thesaurus:	Mycobacterium Bovine tuberculosis Species identification Mycobacterium smegmatis
Keywords:	MALDI-TOF mass spectrometry
Type of Material:	Resumo em anais e proceedings
Access:	openAccess
Appears in Collections:	Resumo em anais de congresso (CNPGC)