Título:	Characterization and genetic variability of coat protein genes of Apple chlorotic leaf spot virus isolates from southern Brazil.
Autor:	NICKEL, O. SILVA, F. N FAJARDO, T. V. M. GORAYEB, E. S.
Afiliación:	OSMAR NICKEL, CNPUV; Fabio N. Silva, Programa de Pós-graduação em Produção Vegetal, Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Lages, SC 88520-000, Brazil; THOR VINICIUS MARTINS FAJARDO, CNPUV; Eduardo E. Gorayeb, Programa de Pós-graduação em Produção Vegetal, Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Lages, SC 88520-000, Brazil.
Año:	2018
Referencia:	Tropical Plant Pathology, v. 43, n. 2, p.109-116, 2018.
Páginas:	8
Descripción:	Apple chlorotic leaf spot virus(ACLSV) infects temperate rosaceous fruit trees worldwide and causes a wide range of diseases that are economically highly damaging. This study was carried out to analyze genetic variability of regional ACLSV isolates and compare it with ACLSV isolates from other parts of the world. Nineteen amplicons of ACLSV, corresponding to the coat protein (CP) gene of isolates from apple, plum, and nectarine, from two states in southern Brazil, have been analyzed for genetic variation and compared phylogenetically among themselves and with other sequences available in GenBank. Sequences identities among complete CP genes of these isolates ranged from 87.5 to 100% and 92.2 to 100% at the nucleotide (nt) and the deduced amino acid (daa) levels, respectively. In the comparison with isolates from Asia, Europe and North America, identities were 68.4 to 100% and 72.5 to 100% at nt and daa levels, respectively. Phylogenetic trees based on nucleotide sequences showed that these isolates grouped into two clusters, cluster 1 containing apple isolates and cluster 2 comprising apple, plum and nec- tarine isolates. Most Brazilian isolates showed conserved signatures (Ser 40 ,Leu 59 ,Tyr 75 ,Thr 130 and Leu 184 )intheirCPs, which place them with type B6 isolates. However, some Brazilian isolates were found to be variants of type B6. These analyzes indicated that Brazilian isolates had lower genetic variability compared to isolates from China, India and Japan and that the CP genes were under negative selection. The greatest diversity of nucleotides was observed in the central portion of the CP gene, represented predominantly by synonymous substitutions. One natural recombinant was detected among ACLSV isolates from Brazil. Keywords ACLSV . Molecular characterization. Variability. Phylogeny. Recombination. Selection.
NAL Thesaurus:	Variability Phylogeny Genetic recombination
Palabras clave:	Molecular characterization Recombination Selection Temperate rosaceous fruit trees ACLSV (Apple chlorotic leaf spot virus) Apple
DOI:	https://doi.org/10.1007/s40858-017-0197-6
Notas:	https://doi.org/10.1007/s40858-017-0197-6
Tipo de Material:	Artigo de periódico
Acceso:	openAccess
Aparece en las colecciones:	Artigo em periódico indexado (CNPUV)