Título:	Genetic Mapping With Allele Dosage Information in Tetraploid Urochloa decumbens (Stapf) R. D. Webster Reveals Insights Into Spittlebug (Notozulia entreriana Berg) Resistance.
Autor:	FERREIRA, R. C. U. LARA, L. A. de C. CHIARI, L. BARRIOS, S. C. L. VALLE, C. B. do VALERIO, J. R. TORRES, F. Z. V. GARCIA, A. A. F. SOUZA, A. P. de
Afiliación:	Rebecca Caroline Ulbricht Ferreira, Universidade de Campinas - Unicamp/Centro de Biologia Molecular e Engenharia Genética; Letícia Aparecida de Castro Lara, Universidade de São Paulo - USP/Escola Superior de Agricultura Luiz de Queiroz - ESALQ/Departameto de Genética; LUCIMARA CHIARI, CNPGC; SANZIO CARVALHO LIMA BARRIOS, CNPGC; CACILDA BORGES DO VALLE, CNPGC; JOSE RAUL VALERIO, CNPGC; FABRICIA ZIMERMANN VILELA TORRES, CNPGC; Antonio Augusto Franco Garcia, Universidade de São Paulo - USP/Escola Superior de Agricultura Luiz de Queiroz - ESALQ/Departameto de Genética; Anete Pereira de Souza, Universidade de Campinas - Unicamp/Centro de Biologia Molecular e Engenharia Genética.
Año:	2019
Referencia:	Frontiers in Plant Science, v. 10, February, 2019.
Descripción:	Urochloa decumbens (Stapf) R. D. Webster is one of the most important African forage grasses in Brazilian beef production. Currently available genetic-genomic resources for this species are restricted mainly due to polyploidy and apomixis. Therefore, crucial genomic-molecular studies such as the construction of genetic maps and the mapping of quantitative trait loci (QTLs) are very challenging and consequently affect the advancement of molecular breeding. The objectives of this work were to (i) construct an integrated U. decumbens genetic map for a full-sibling progeny using GBS-based markers with allele dosage information, (ii) detect QTLs for spittlebug (Notozulia entreriana) resistance, and (iii) seek putative candidate genes involved in defense against biotic stresses. We used the Setaria viridis genome a reference to align GBS reads and selected 4,240 high-quality SNP markers with allele dosage information. Of these markers, 1,000 were distributed throughout nine homologous groups with a cumulative map length of 1,335.09 cM and an average marker density of 1.33 cM. We detected QTLs for resistance to spittlebug, an important pasture insect pest, that explained between 4.66 and 6.24% of the phenotypic variation. These QTLs are in regions containing putative candidate genes related to defense against biotic stresses. Because this is the first genetic map with SNP autotetraploid dosage data and QTL detection in U. decumbens, it will be useful for future evolutionary studies, genome assembly, and other QTL analyses in Urochloa spp. Moreover, the results might facilitate the isolation of spittlebug-related candidate genes and help clarify the mechanism of spittlebug resistance. These approaches will improve selection efficiency and accuracy in U. decumbens molecular breeding and shorten the breeding cycle.
Thesagro:	Brachiaria
NAL Thesaurus:	Polyploidy Quantitative traits
Palabras clave:	Allele dosage Linkage map Signalgrass SNP
Tipo de Material:	Artigo de periódico
Acceso:	openAccess
Aparece en las colecciones:	Artigo em periódico indexado (CNPGC)