Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1107120
Research center of Embrapa/Collection: Embrapa Agroenergia - Artigo em periódico indexado (ALICE)
Issue Date: 2019
Type of Material: Artigo em periódico indexado (ALICE)
Authors: MESQUITA, L. M. de S.
RODRIGUES, C. F. B.
ROCHA, C. Q. da
BIANCHIM, M. S.
RODRIGUES, C. M.
OLIVEIRA, V. de
GAETA, H. H.
BELCHOR, M. N.
TOYAMA. M. H.
VILEGAS, W.
Additional Information: Leonardo Mendes de Souza Mesquita; Caroline Fabri Bittencourt Rodrigues; Claudia Quintino da Rocha; Mayara Silveira Bianchim; CLENILSON MARTINS RODRIGUES, CNPAE; Vanda Maria de Oliveira; Henrique Hessel Gaeta; Mariana Novo Belchor; Marcos Hikari Toyama; Wagner Vilegas.
Title: LC-ESI-IT-MS/MS and MALDI-TOF approach: identification of natural polymers from rhizophora mangle barks and determination of their analgesic and anti-inflammatory properties.
Publisher: Natural Products and Bioprospecting, v. 9, p. 23-34, 2019.
Language: en
Keywords: Morphine-like effect
Catechins
Anti-inflammatory
Mangroves
Description: We recognize the chemical composition of the acetonic extract of Rhizophora mangle barks (AERM) using mass spectrometry analysis [liquid chromatography (LC)?ESI?IT-MS/MS and matrix-assisted laser desorption/ionization-time of flight-MS (MALDI-TOF)]. Analgesic activity was evaluated by formalin and tail-flick experimental assays. Anti-inflammatory activity was performed by paw edema test induced by carrageenan and 48/80 compounds. The first series of experiments involved [LC]?FIA?IT-MS/MS with 11 separated catechins derivatives until degree of polymerization 3 (DP3). The spectra obtained by MALDI-TOF analysis of the AERM presented two homologous series: one based on polymers of m/z 288 Da increments (up to DP12) and another series based on polymers of m/z [288 ? 162] Da increments (up to DP11). In addition to these series of flavan-3-ol, each DP had a subset of masses with a variation of - 16 Da (homologous series of afzelechins?m/z 873?3465 Da) and ? 16 Da (homologous series of gallocatechins?m/z 905?3497 Da). A similar pattern with homologous series of gallocatechins and afzelechins could also be observed for a fifth and a sixth monohexoside series: glucogallocatechins (m/z 779?3371) and glucoafzelechins (m/z 747?3339). The intraperitoneal administration of different doses of AERM (50, 150 and 300 lg mL-1) have a morphine-like effect and intense anti-inflammatory activity.
Year: 2019-03-15
Appears in Collections:Artigo em periódico indexado (CNPAE)

Files in This Item:
File Description SizeFormat 
CLENILSON041NaturalProductsandBioprospecting.pdf1,1 MBAdobe PDFThumbnail
View/Open

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace