Title:	Co-expression networks reveal potential regulatory roles of miRNAs in fatty acid composition of nelore cattle.
Authors:	OLIVEIRA, P. S. N. de COUTINHO, L. L. CESAR, A. S. M. DINIZ, W. J. da S. SOUZA, M. de S. ANDRADE, B. G. KOLTES, J. E. MOURÃO, G. B. ZERLOTINI NETO, A. REECY, J. M. REGITANO, L. C. de A.
Affiliation:	PRISCILA S. N. DE OLIVEIRA, CPPSE; LUIZ L. COUTINHO, Esalq/USP; ALINE S. M. CESAR, Esalq/USP; WELLISON J. DA SILVA DINIZ, UFSCar; MARCELA M. DE SOUZA, Iowa State University; BRUNO G. ANDRADE, CPPSE; JAMES E. KOLTES, Iowa State University; GERSON B. MOURÃO, Esalq/USP; ADHEMAR ZERLOTINI NETO, CNPTIA; JAMES M. REECY, Iowa State University; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.
Date Issued:	2019
Citation:	Frontiers in Genetics, v. 10, p. 1-14, July 2019.
Description:	Fatty acid (FA) content affects the sensorial and nutritional value of meat and plays a significant role in biological processes such as adipogenesis and immune response. It is well known that, in beef, the main FAs associated with these biological processes are oleic acid (C18:1 cis9, OA) and conjugated linoleic acid (CLA-c9t11), which may have beneficial effects on metabolic diseases such as type 2 diabetes and obesity. Here, we performed differential expression and co-expression analyses, weighted gene co-expression network analysis (WGCNA) and partial correlation with information theory (PCIT), to uncover the complex interactions between miRNAs and mRNAs expressed in skeletal muscle associated with FA content. miRNA and mRNA expression data were obtained from skeletal muscle of Nelore cattle that had extreme genomic breeding values for OA and CLA. Insulin and MAPK signaling pathways were identified by WGCNA as central pathways associated with both of these fatty acids. Co-expression network analysis identified bta-miR-33a/b, bta-miR-100, bta-miR-204, bta-miR-365-5p, btamiR-660, bta-miR-411a, bta-miR-136, bta-miR-30-5p, bta-miR-146b, bta-let-7a-5p, bta-let-7f, bta-let-7, bta-miR 339, bta-miR-10b, bta-miR 486, and the genes ACTA1 and ALDOA as potential regulators of fatty acid synthesis. This study provides evidence and insights into the molecular mechanisms and potential target genes involved in fatty acid content differences in Nelore beef cattle, revealing new candidate pathways of phenotype modulation that could positively benefit beef production and human consumption.
Thesagro:	Bos Indicus Gado de Corte
NAL Thesaurus:	Beef cattle Conjugated linoleic acid Oleic acid
Keywords:	Ácido linoleico conjugado Ácido oleico Ácidos graxos Genômica Redes de co-expressão Integrative genomics MRNA MiRNA
DOI:	10.3389/fgene.2019.00651
Notes:	Article 651. Na publicação: Adhemar Zerlotini, Luciana C.A. Regitano.
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPTIA)