Title:	Effects of nitrogen and phosphorus on biochemical composition of microalgae polyculture harvested from phycoremediation of piggery wastewater digestate.
Authors:	MICHELON, W. SILVA, M. L. B. da MEZZARI, M. P. PIROLLI, M. PRANDINI, J. M. SOARES, H. M.
Affiliation:	MARCIO LUIS BUSI DA SILVA, CNPSA; MELISSA PAOLA MEZZARI.
Date Issued:	2016
Citation:	Applied Biochemistry and Biotechnology, v. 178, n. 7, p. 1407-1419, 2016.
Description:	Abstract: The effects of nitrogen (N) and/or phosphorus (P) starvation on the biochemical composition of native microalgae Chlorella spp. polyculture obtained from the phycoremediation of swine wastewaters were investigated. Microalgae-specific growth rate of 1.2 day(-1) was achieved (30.3 mg L(-1) day(-1)). PO4 (-2) and NH3 were completely removed from swine digestate effluent after 3 and 11 days, respectively. Microalgae harvested immediately after nutrient removal showed high protein (56-59 %) and carbohydrate (25-34 %) but low lipid (1.8-3 %) contents. Depletion of N or P alone stimulated carbohydrate production at the expenses of proteins. Significant lipid accumulation from 3 %&#8201;±&#8201;0.5 to 16.3 %&#8201;±&#8201;0.8 was reached only after 25 days following N and P starvation as demonstrated by Nile red-stained cells. Regarding to the effects of harvesting methods on cellular biochemical composition, circumstantial evidences indicate that coagulation-flocculation with tannin may lead to lower protein and lipid amounts but increased carbohydrate content (p&#8201;<&#8201;0.01) as compared to centrifugation.
Thesagro:	Suíno Microalga Águas Residuais
NAL Thesaurus:	Carbohydrates Lipids Microalgae
Keywords:	Swine wastewater Phycoremediation
DOI:	10.1007/s12010-015-1955-x
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPSA)