Título:	Associative diazotrophic bacteria from forage grasses in theBrazilian semi-arid region are effective plant growth promoters.
Autoria:	ANTUNES, G. dos R. SANTANA, S. R. A. ESCOBAR, I. E. C. BRASIL, M. da S. ARAUJO, G. G. L. de VOLTOLINI, T. V. FERNANDES JUNIOR, P. I.
Afiliação:	Gabiane dos Reis Antunes, UNIVASF; Sheilla Rios Assis Santana, UNIVASF; Indra Elena Costa Escobar, UNIVASF; Marivaine da Silva Brasil, Universidade Federal do Mato Grosso do Sul, Corumbá, MS; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; TADEU VINHAS VOLTOLINI, CPATSA; PAULO IVAN FERNANDES JUNIOR, CPATSA.
Ano de publicação:	2019
Referência:	Crop & Pasture Science, v. 70, p. 899-907, 2019.
Conteúdo:	The study of plant growth-promoting bacteria (PGPB) can identify outstanding bacteria for crops. For forage grasses adapted to drylands, the selection of PGPB can increase the field performance of pastures. The aim of this study was to isolate, and characterise at molecular, biochemical and symbiotic levels, diazotrophic bacteria obtained from buffel grass (Cenchrus ciliaris), sorghum (Sorghum bicolor) and Tifton 85 (Cynodon spp.) from Brazilian semi-arid region fields. Fieldgrown plants were collected, and the roots were surface-disinfected, crushed and inoculated in a semi-solid medium. After the formation and confirmation of microaerophilic pellicles, the bacteria were isolated and purified. All bacterial isolates were subjected to nifH gene amplification and identified by their partial 16S rRNA gene sequences. The bacteria were evaluated for the production of auxins and siderophores, calcium phosphate solubilisation, and diazotrophic ability as ?in vitro? plant growth-promotion traits. A plant inoculation assay was conducted to assess the plant growth promotion abilities of the bacterial isolates. Twenty-one bacterial isolates harboured the nifH gene (nifH+), among which nine were obtained from sorghum, eight from buffel grass, and four from Tifton 85. The bacterial isolates were classified as Bacillus (8), Stenotrophomonas (7), Agrobacterium (4), Cellulomonas (1) and Paenibacillus (1). All were shown to be auxin producers, with 14 isolates showing diazotrophic capacity ?in vitro?. Fourteen isolates increased plant N content, but the bacterial strains ESA 392 and ESA 398 (Bacillus), ESA 397 and ESA 407 (Stenotrophomonas), and ESA 401 (Agrobacterium) were shown to promote both plant growth and N nutrition. These strains are candidates for further assays to evaluate their agronomic performance under field conditions, aiming inoculant production for forage grasses in drylands.
Thesagro:	Gramínea Forrageira Caatinga Bactéria Capim Buffel Sorgo Forragem
NAL Thesaurus:	Forage grasses
Palavras-chave:	Semiárido Bactérias diazotróficas Crescimento vegetal Seleção de linhagem Fixação biológica de nitrogênio PGPB
Digital Object Identifier:	10.1071/CP19076
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPATSA)