Title:	Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content.
Authors:	CARVALHO, B. P. COSTA, F. de Q. DETONI, D. ROSA, F. B. DIAS, A. J. B.
Affiliation:	BRUNO PENA CARVALHO, CPAF-AC; Fernanda de Queirós Costa, Universidade Estadual do Norte Fluminense Darcy Ribeiro; Danielly Detoni, Universidade Estadual do Norte Fluminense Darcy Ribeiro; Felipe Borges Rosa, Universidade Estadual do Norte Fluminense Darcy Ribeiro; Angelo José Burla Dias, Universidade Estadual do Norte Fluminense Darcy Ribeiro.
Date Issued:	2019
Citation:	Revista Brasileira de Zootecnia, v. 48, e20180322, set. 2019.
Description:	This study evaluated the effect of the addition of conjugated linoleic acid (CLA) to in vitro culture on viability, lipid content, and cryoresistance of bovine embryos at different in vitro culture times. Cumulus oocyte complexes (N = 974) were maturated in vitro for 22 h. In vitro fecundation ensued for 18 h. Viable zygotes were cultivated in vitro in medium supplemented with CLA (100 mM) in the first 72 h (CLA-F), last 72 h (CLA-L), or throughout the culture period (CLA-T). Control embryos (control) were cultivated with no CLA. Embryos were cryopreserved by vitrification for subsequent analysis after devitrification. Effect of CLA on cryoresistance was assessed by cultivating embryos in synthetic oviductal fluid containing 5% fetal bovine serum. Lipid content was quantified using Nile Red staining. No significant difference was observed in cleavage rate, blastocyst:total oocyte ratio, and blastocyst:cleaved oocyte ratio. Culture in CLA-L reduced survival rate 24 h after devitrification compared with CLA-F and CLA-T, although with no statistically significant difference compared with control group. However, CLA-T improved embryo hatching rate and affected lipid content of embryos. Cultures CLA-F and CLA-L increased lipid content compared with control. Yet, lipid content values decreased in embryos treated with CLA-T, but they did not differ significantly from the values observed for oocytes at the germinal vesicle stage. Treatment of bovine embryos with CLA during in vitro cultivation did not affect the production of blastocysts, reducing lipid content and improving cryoresistance. However, the effects of CLA on cryoresistance and lipid content is significant only when embryos are exposed to the compound throughout the cultivation period.
Thesagro:	Bovino Embrião Animal Cultura In Vitro Lipídio Reprodução Animal Resistência a Temperatura Congelamento Criopreservação
NAL Thesaurus:	Embryo (animal) Cattle Animal reproduction Conjugated linoleic acid Polyunsaturated fatty acids Cryopreservation Cold tolerance Embryo culture Lipid content
Keywords:	Reproducción de animales Ganado bovino Embrión (animal) Cultivo de embriones Contenido de lípidos Ácido linoleíco conjugado Ácido graxo poliinsaturado Ácidos grasos polisaturados Oocyte vitrification Vitrificación de ovocitos Criopreservación Tolerancia al frío
ISSN:	1806-9290
DOI:	10.1590/rbz4820180322
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPAF-AC)