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dc.contributor.authorGARCIA, A. R.pt_BR
dc.contributor.authorMOURA, A. B. B.pt_BR
dc.contributor.authorROMANELLO, N.pt_BR
dc.contributor.authorFONSECA, J. F. dapt_BR
dc.contributor.authorBRANDÃO, F. Z.pt_BR
dc.contributor.authorPANTOJA, M. H. de A.pt_BR
dc.contributor.authorBARRETO, A. do N.pt_BR
dc.contributor.authorSOUSA, M. A. de P.pt_BR
dc.contributor.authorBOTTA, D.pt_BR
dc.contributor.authorGIRO, A.pt_BR
dc.date.accessioned2019-12-12T18:17:04Z-
dc.date.available2019-12-12T18:17:04Z-
dc.date.created2019-12-12
dc.date.issued2019
dc.identifier.citationIn: REUNIÃO ANUAL DA ASSOCIAÇÃO BRASILEIRA DE ANDROLOGIA ANIMAL (ABRAA), 4., 2019, Goiânia. Anais... Goiânia: Editora UFMS, 2019.pt_BR
dc.identifier.issn978-85-0000-000-0pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1116740-
dc.descriptionThe study aimed to establish the most suitable osmolarity range of the hypoosmotic solution for testing the membrane integrity in raw semen of rams. Sodium citrate and fructose based solutions (S) with the following osmolarities (mOsm/L) were used: 50 (S1), 75 (S2), 100 (S3), 125 (S4), 150 (S5), 175 (S6), 200 (S7), 250 (S8), 290 (S9) and 300 mOsm/L (S10). Nine mature Santa Ines rams (21.5+0.5 months; 72.9+1.6 kg) had semen collected one month apart. After the hypoosmotic swelling test (HOST) the ejaculates were evaluated using a phase-contrast microscope. A total of 200 spermatozoa were counted and sperm cells were classified as spermatozoa with coiled tails (CD, %), strongly coiled tail (CFD, %) or non-coiled tails. According to CD, S1, S2, S3, S4, S5, S6 and S7 were superior to S8, S9 and S10 (P<0,05). According to CED, S2, S3, S4, S5, S6 and S7 were superior to S8, S9 and S10 (P<0,05). Results suggest that the 75 up to 200 mOsm/L solution would be bestfor using in HOST when raw semen of rams is analyzed.pt_BR
dc.language.isoporpt_BR
dc.rightsopenAccesspt_BR
dc.subjectSolução hiposmóticapt_BR
dc.subjectHypoosmotic solutionpt_BR
dc.subjectRampt_BR
dc.subjectSpermpt_BR
dc.titleOsmolaridade ideal para execução do teste hiposmótico no sêmen in natura de ovinos (Ovis aries).pt_BR
dc.typeArtigo em anais e proceedingspt_BR
dc.date.updated2020-01-07T11:11:11Z
dc.subject.thesagroOvinopt_BR
dc.subject.thesagroEspermatozóidept_BR
dc.description.notes7 e 8 de junho de 2019.pt_BR
dc.format.extent2p.160-163.pt_BR
riaa.ainfo.id1116740pt_BR
riaa.ainfo.lastupdate2020-01-07 -02:00:00
dc.contributor.institutionALEXANDRE ROSSETTO GARCIA, CPPSE; Ana Beatriz Bossois Moura, UFF; Narian Romanello, USP; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Zandonadi Brandão, UFF; Messy Hannear de Andrade Pantoja, USP; Andréa do Nascimento Barreto, UFPA; Marco Antonio de Paula Sousa, UFPA; Daniela Botta, UFPA; Alessandro Giro, UFPA.pt_BR
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